Key features and details
- Mouse monoclonal [M232] to eNOS (phospho S632)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-eNOS (phospho S632) antibody [M232]
See all eNOS primary antibodies
DescriptionMouse monoclonal [M232] to eNOS (phospho S632)
Specificityab76199 is not expected to react with other NOS family members due to low homology.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic phosphopeptide corresponding to amino acids surrounding Ser632 in mouse eNOS, coupled to carrier protein. This sequence is conserved in human (Ser633) and rat (Ser632) eNOS.
- Human umbilical vein endothelial cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab76199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa).|
FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Tissue specificityPlatelets, placenta, liver and kidney.
Involvement in diseaseVariation in NOS3 seem to be associated with susceptibility to coronary spasm.
Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
modificationsPhosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
Cellular localizationCell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
- Information by UniProt
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution
Lane 1 : human umbilical vein endothelial
Lane 2 : human umbilical vein endothelial
cells, treated with lambda phosphatase
Predicted band size: 133 kDa
Observed band size: 120,140 kDa why is the actual band size different from the predicted?
The 120 kDa band may be a truncated form of eNOS.
ab76199 has been referenced in 11 publications.
- Xu H et al. GATA4 protects against hyperglycemia-induced endothelial dysfunction by regulating NOX4 transcription. Mol Med Rep 17:1485-1492 (2018). PubMed: 29138836
- Zhou Q et al. Effects of astragalus polysaccharide on apoptosis of myocardial microvascular endothelial cells in rats undergoing hypoxia/reoxygenation by mediation of the PI3K/Akt/eNOS signaling pathway. J Cell Biochem 119:806-816 (2018). PubMed: 28657671
- Liu LJ et al. Kappa-opioid receptor agonist U50448H protects against renal ischemia-reperfusion injury in rats via activating the PI3K/Akt signaling pathway. Acta Pharmacol Sin 39:97-106 (2018). PubMed: 28770825
- Gupta A et al. PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Mol Cell 65:999-1013.e7 (2017). PubMed: 28306514
- Yang HH et al. Protective Effects of MicroRNA-126 on Human Cardiac Microvascular Endothelial Cells Against Hypoxia/Reoxygenation-Induced Injury and Inflammatory Response by Activating PI3K/Akt/eNOS Signaling Pathway. Cell Physiol Biochem 42:506-518 (2017). PubMed: 28578351