Overview

  • Product name

    Anti-eNOS (phospho S632) antibody [M232]
    See all eNOS primary antibodies
  • Description

    Mouse monoclonal [M232] to eNOS (phospho S632)
  • Host species

    Mouse
  • Specificity

    ab76199 is not expected to react with other NOS family members due to low homology.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acids surrounding Ser632 in mouse eNOS, coupled to carrier protein. This sequence is conserved in human (Ser633) and rat (Ser632) eNOS.

  • Positive control

    • Human umbilical vein endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab76199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa).
ELISA 1/1000.

Target

  • Function

    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • Tissue specificity

    Platelets, placenta, liver and kidney.
  • Involvement in disease

    Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • Sequence similarities

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Post-translational
    modifications

    Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • Cellular localization

    Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Images

  • All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution

    Lane 1 : human umbilical vein endothelial
    cells, untreated
    Lane 2 : human umbilical vein endothelial
    cells, treated with lambda phosphatase

    Predicted band size: 133 kDa
    Observed band size: 120,140 kDa
    why is the actual band size different from the predicted?



    The 120 kDa band may be a truncated form of eNOS.

References

This product has been referenced in:

  • Xu H  et al. GATA4 protects against hyperglycemia-induced endothelial dysfunction by regulating NOX4 transcription. Mol Med Rep 17:1485-1492 (2018). Read more (PubMed: 29138836) »
  • Zhou Q  et al. Effects of astragalus polysaccharide on apoptosis of myocardial microvascular endothelial cells in rats undergoing hypoxia/reoxygenation by mediation of the PI3K/Akt/eNOS signaling pathway. J Cell Biochem 119:806-816 (2018). Read more (PubMed: 28657671) »
See all 11 Publications for this product

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