Key features and details
- Rabbit polyclonal to ENOX2/tNOX
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ENOX2/tNOX antibody
DescriptionRabbit polyclonal to ENOX2/tNOX
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human ENOX2/tNOX aa 411-610 (C terminal).
Database link: Q16206
- WB: MCF-7, THP-1, SW480, Jurkat, SKOV3 cell lysates, mouse liver, kidney and ovary, rat liver tissue lysates; ICC/IF: C6 cells.
Previously labelled as ENOX2.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 70 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionMay be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
Tissue specificityFound in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells. Found on the cell borders of renal, kidney and ovarian carcinomas but not on the borders of surrounding non-cancerous stromal cells.
Sequence similaritiesBelongs to the ENOX family.
Contains 1 RRM (RNA recognition motif) domain.
Cellular localizationCell membrane. Secreted > extracellular space. Extracellular and plasma membrane-associated.
- Information by UniProt
- APK1 antibody
- APK1 antigen antibody
- COVA1 antibody
Immunofluorescence staining of C6 cells stained for ENOX2/tNOX with ab210751 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-ENOX2/tNOX antibody (ab210751)
Lane 1 : MCF-7 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : SKOV3 cell lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Mouse ovary tissue lysate
Lane 9 : Rat liver tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 15 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
ab210751 has not yet been referenced specifically in any publications.