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a customer of ours would like to perform a sandwich ELISA by using antibody pairs and she would like to use as polyclonal Ab AB82383.
Now she would like to know which is the best monoclonal Ab (between AB77104andAB8259) to along with AB82383 to perform an ELISA.
Thanks in advance
Asked on Feb 10 2012
Thank you for your enquiry.
I am sorry to confirm that these antibodies have not been tested in sandwich ELISA (sELISA). They have been tested in indirect ELISA only and I can recommend considering using them in an indirect ELISA rather than sELISA.
Sandwich ELISA procedures can be difficult to optimise and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, I am sorry we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA.
I am sorry I am unable to suggest any other antibodies suitable for sandwich ELISA for you on this occasion. However, I hope this information is helpful, Should you have any further questions, please do not hesitate to contact us.
Answered on Feb 10 2012