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Hi,thank you for your last letter,I use your product primary antibody (Anti-ENPP2 antibody [5H3] (ab77104)),i am doing western blotting experiment,but your product instruction book do not clear,Should I use SDS-PAGE gel(sodium dodecyl sulfate–polyacrylamide gel electrophoresis)or no use SDS ?I want to known your Positive control is how to do ? i need your experiments Detailed steps and requirements.
Asked on Apr 16 2012
Thank you for contacting us.
We recommend to run the gel in denaturing and reducing conditions for ab77104. Therefore, SDS PAGE gel should be used. Attached is our WB protocol and you could use it as a reference.
We use HEK 293 (huamn embryonic kidney cell line) as positive control. You could prepare the cell lysate according to the attached protocol or use ab7902 (HEK293 Human embryonic kidney cell line Whole Cell Lysate) as positive control.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Apr 16 2012