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The protein is 50-100ug per lane, and the primary ENPP2 was diluted 1:200, 1:400 and so on. We repeated many times. We used BSA as blocking recently,but the films is blank.The sample have signals with other antibody such as actin. The secondary was fine because we used for other primary antibody. There were many papers showing HEK 293 lysates has high level of ENPP2. It is better if we can have the replacement of the same Cat #ab77104.
Asked on Sep 18 2012
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. This will be the same lot as what you received before. We do not have other lots, but we have also not received other complaints about this one, and it is possible the issue was with the single vial.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Sep 18 2012