Anti-ENPP2/ATX antibody (ab137590)
Key features and details
- Rabbit polyclonal to ENPP2/ATX
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ENPP2/ATX antibody
See all ENPP2/ATX primary antibodies -
Description
Rabbit polyclonal to ENPP2/ATX -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ENPP2/ATX aa 540-803.
Database link: Q13822 -
Positive control
- WB: Mouse brown adipose tissue, HepG2 and MOLT-4 whole cell lysate. Rat and mouse brain. Human Cerebrospinal Fluid. ICC/IF: SK-N-SH cells.
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General notes
This product was previously labelled as ENPP2
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 105 kDa.
|
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| ICC/IF | (1) |
1/100 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 105 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. -
Tissue specificity
Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. -
Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains. -
Post-translational
modificationsN-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. -
Cellular localization
Secreted. Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells. - Information by UniProt
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Database links
- Entrez Gene: 5168 Human
- Omim: 601060 Human
- SwissProt: Q13822 Human
- Unigene: 190977 Human
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Alternative names
- ATX antibody
- ATX X antibody
- Autotaxin antibody
see all
Images
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Western blot - Anti-ENPP2/ATX antibody (ab137590)Anti-ENPP2/ATX antibody (ab137590) at 1/1000 dilution + Human Cerebrospinal Fluid at 30 µg
Predicted band size: 105 kDa7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (ab137590)SK-N-SH cells stained for Autotaxin (green) using ab137590 at 1/500 dilution in ICC/IF.
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Western blot - Anti-ENPP2/ATX antibody (ab137590)All lanes : Anti-ENPP2/ATX antibody (ab137590) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 105 kDa
7.5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137590 has been referenced in 1 publication.
- Seo EJ et al. Autotaxin Regulates Maintenance of Ovarian Cancer Stem Cells through Lysophosphatidic Acid-Mediated Autocrine Mechanism. Stem Cells 34:551-64 (2016). PubMed: 26800320