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    enpp2atx-antibody-ab137590.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Lipases
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Anti-ENPP2/ATX antibody (ab137590)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-ENPP2/ATX antibody (ab137590)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (ab137590)
  • Western blot - Anti-ENPP2/ATX antibody (ab137590)

Key features and details

  • Rabbit polyclonal to ENPP2/ATX
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Primary
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Anti-IL-8 antibody (ab106350)
Protein
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Recombinant Human ENPP2/ATX protein (ab126918)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ENPP2/ATX antibody
    See all ENPP2/ATX primary antibodies
  • Description

    Rabbit polyclonal to ENPP2/ATX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human ENPP2/ATX aa 540-803.
    Database link: Q13822

  • Positive control

    • WB: Mouse brown adipose tissue, HepG2 and MOLT-4 whole cell lysate. Rat and mouse brain. Human Cerebrospinal Fluid. ICC/IF: SK-N-SH cells.
  • General notes

     This product was previously labelled as ENPP2

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human ENPP2/ATX protein (ab126918)
    • Raji whole cell lysate (ab30124)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human ENPP2/ATX protein (ab126918)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 105 kDa.
ICC/IF (1)
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 105 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.
  • Tissue specificity

    Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.
  • Sequence similarities

    Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
    Contains 2 SMB (somatomedin-B) domains.
  • Post-translational
    modifications

    N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity.
  • Cellular localization

    Secreted. Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells.
  • Target information above from: UniProt accession Q13822 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5168 Human
    • Omim: 601060 Human
    • SwissProt: Q13822 Human
    • Unigene: 190977 Human
    • Alternative names

      • ATX antibody
      • ATX X antibody
      • Autotaxin antibody
      • Autotaxin t antibody
      • E NPP 2 antibody
      • E-NPP 2 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase 2 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 antibody
      • Enpp2 antibody
      • ENPP2_HUMAN antibody
      • Extracellular lysophospholipase D antibody
      • FLJ26803 antibody
      • LysoPLD antibody
      • NPP2 antibody
      • PD IALPHA antibody
      • PDNP2 antibody
      • Phosphodiesterase I alpha antibody
      • Phosphodiesterase I/nucleotide pyrophosphatase 2 antibody
      • Plasma lysophospholipase D antibody
      see all

    Images

    • Western blot - Anti-ENPP2/ATX antibody (ab137590)
      Western blot - Anti-ENPP2/ATX antibody (ab137590)
      Anti-ENPP2/ATX antibody (ab137590) at 1/1000 dilution + Human Cerebrospinal Fluid at 30 µg

      Predicted band size: 105 kDa



      7.5% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (ab137590)
      Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (ab137590)

      SK-N-SH cells stained for Autotaxin (green) using ab137590 at 1/500 dilution in ICC/IF. 

    • Western blot - Anti-ENPP2/ATX antibody (ab137590)
      Western blot - Anti-ENPP2/ATX antibody (ab137590)
      All lanes : Anti-ENPP2/ATX antibody (ab137590) at 1/500 dilution

      Lane 1 : HepG2 whole cell lysate
      Lane 2 : MOLT4 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 105 kDa



      7.5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab137590? Please let us know so that we can cite the reference in this datasheet.

    ab137590 has been referenced in 1 publication.

    • Seo EJ  et al. Autotaxin Regulates Maintenance of Ovarian Cancer Stem Cells through Lysophosphatidic Acid-Mediated Autocrine Mechanism. Stem Cells 34:551-64 (2016). PubMed: 26800320

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ENPP2/ATX antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Vascular smooth muscle cells)
    Permeabilization
    Yes - Triton
    Specification
    Vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 21 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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