Key features and details
- Goat polyclonal to ENPP2/ATX
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ENPP2/ATX antibody
See all ENPP2/ATX primary antibodies
DescriptionGoat polyclonal to ENPP2/ATX
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Monkey, Gorilla, Bat
- Human Brain, Cortex, and liver tissue; Human Placenta lysate
This product was previously labelled as ENPP2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab191272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionHydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.
Tissue specificityPredominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.
Sequence similaritiesBelongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains.
modificationsN-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity.
Cellular localizationSecreted. Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells.
- Information by UniProt
- ATX antibody
- ATX X antibody
- Autotaxin antibody
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue labeling ENPP2/ATX using ab191272 at 5 µg/ml.
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain, cortex, tissue labeling ENPP2/ATX using ab191272 at 5 µg/ml.
Anti-ENPP2/ATX antibody (ab191272) at 0.3 µg/ml + Human placenta lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
ab191272 has not yet been referenced specifically in any publications.