Overview

  • Product name

  • Description

    Goat polyclonal to ENPP2/ATX
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to Human ENPP2/ATX aa 646-660 (internal sequence).
    Sequence:

    PDHLTSCVRPDVRVS


    Database link: Q13822

  • Positive control

    • Human Brain, Cortex, and liver tissue; Human Placenta lysate
  • General notes

     This product was previously labelled as ENPP2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab191272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.
  • Tissue specificity

    Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.
  • Sequence similarities

    Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
    Contains 2 SMB (somatomedin-B) domains.
  • Post-translational
    modifications

    N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity.
  • Cellular localization

    Secreted. Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATX antibody
    • ATX X antibody
    • Autotaxin antibody
    • Autotaxin t antibody
    • E NPP 2 antibody
    • E-NPP 2 antibody
    • Ectonucleotide pyrophosphatase/phosphodiesterase 2 antibody
    • Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 antibody
    • Enpp2 antibody
    • ENPP2_HUMAN antibody
    • Extracellular lysophospholipase D antibody
    • FLJ26803 antibody
    • LysoPLD antibody
    • NPP2 antibody
    • PD IALPHA antibody
    • PDNP2 antibody
    • Phosphodiesterase I alpha antibody
    • Phosphodiesterase I/nucleotide pyrophosphatase 2 antibody
    • Plasma lysophospholipase D antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue labeling ENPP2/ATX using ab191272 at 5 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain, cortex, tissue labeling ENPP2/ATX using ab191272 at 5 µg/ml.

  • Anti-ENPP2/ATX antibody (ab191272) at 0.3 µg/ml + Human placenta lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 99 kDa

References

ab191272 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191272.
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