Key features and details
- Mouse monoclonal [4C1H2] to ENPP3/B10
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ENPP3/B10 antibody [4C1H2]
See all ENPP3/B10 primary antibodies
DescriptionMouse monoclonal [4C1H2] to ENPP3/B10
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: Human recombinant ENPP3/B10 (AA: extra 45-163) protein; ENPP3/B10 (AA: extra 45-163)-hIgGFc transfected HEK-293 cell lysate. Flow: HL-60 cells. IHC-P: Human renal cancer tissues.
This product was previously labelled as ENPP3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|IHC-P||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.|
FunctionCleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
Tissue specificityExpressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
Sequence similaritiesBelongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains.
modificationsN-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Cellular localizationMembrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
- Information by UniProt
- Alkaline phosphodiesterase I antibody
- ARHR2 antibody
- B10 antibody
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling ENPP3/B10 using ab233777 at 1/200 dilution (green) and the negative control (red).
Paraffin-embedded human renal cancer tissue stained for ENPP3/B10 using ab233777 at 1/200 dilution in immunohistochemical analysis. DAB staining.
All lanes : Anti-ENPP3/B10 antibody [4C1H2] (ab233777) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : ENPP3/B10 (AA: extra 45-163)-hIgGFc transfected HEK-293 cell lysate.
Anti-ENPP3/B10 antibody [4C1H2] (ab233777) at 1/500 dilution + Human recombinant ENPP3/B10 (AA: extra 45-163) protein.
Expected MW is 13.6 kDa.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233777 has been referenced in 1 publication.
- Sakamoto S et al. Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples. Sci Adv 6:eaay0888 (2020). PubMed: 32195342