Key features and details
- Rabbit polyclonal to ENPP3/B10
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ENPP3/B10 antibody
See all ENPP3/B10 primary antibodies
DescriptionRabbit polyclonal to ENPP3/B10
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ENPP3/B10 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: NP_005012.2
- Human ovary and testis tissues.
This product was previously labelled as ENPP3
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab118445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 15 - 20 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.|
FunctionCleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
Tissue specificityExpressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
Sequence similaritiesBelongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains.
modificationsN-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Cellular localizationMembrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
- Information by UniProt
- Alkaline phosphodiesterase I antibody
- ARHR2 antibody
- B10 antibody
All lanes : Anti-ENPP3/B10 antibody (ab118445) at 1/1000 dilution
Lane 1 : HEK293 cell lysate - untransfected
Lane 2 : HEK293 cell lysate - transfected with ENNP3
Lane 3 : HEK293 cell lysate - transfected with ENPP1
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab118445 at 20µg/ml staining ENPP3/B10 in Formalin-fixed, Paraffin-embedded Human ovary tissue by immunohistochemistry.
ab118445 at 20µg/ml staining ENPP3/B10 in Formalin-fixed, Paraffin-embedded Human testis tissue by immunohistochemistry.
ab118445 has not yet been referenced specifically in any publications.