PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)
Key features and details
- PE Mouse monoclonal [NP4D6] to ENPP3/B10
- Suitable for: Flow Cyt, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-ENPP3/B10 antibody [NP4D6]
See all ENPP3/B10 primary antibodies -
Description
PE Mouse monoclonal [NP4D6] to ENPP3/B10 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human ENPP3/B10. HEK-293 cells transfected with human ENPP3/B10
Database link: O14638 -
Positive control
- Flow Cyt: human basophils. Flow Cyt (Intra): human blood cells ICC/IF: human basophils
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General notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
NP4D6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | (2) |
Use 20µl for 106 cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF | (1) |
Use at an assay dependent concentration.
|
Flow Cyt (Intra) |
Use 20µl for 106 cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 20µl for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use 20µl for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. -
Tissue specificity
Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors. -
Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains. -
Post-translational
modificationsN-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. -
Cellular localization
Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 5167 Human
- Entrez Gene: 5169 Human
- Omim: 602182 Human
- SwissProt: O14638 Human
- SwissProt: P22413 Human
- Unigene: 486489 Human
- Unigene: 527295 Human
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Alternative names
- Alkaline phosphodiesterase I antibody
- ARHR2 antibody
- B10 antibody
see all
Images
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ab90751 staining ENPP3/B10 on the surface of human basophils isolated from allergen-stimulated whole blood by flow cytometery.
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Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3/B10 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab90751 has not yet been referenced specifically in any publications.