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Neuroscience Neurotransmission Secretory Vesicles Regulation
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RecombinantRabMAb

Recombinant Anti-ENPP5 antibody [EPR8236] (ab137014)

  • Datasheet
  • SDS
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Product price, shipping and contact information

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Western blot - Anti-ENPP5 antibody [EPR8236] (ab137014)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENPP5 antibody [EPR8236] (ab137014)
  • OI-RD Scanning - Anti-ENPP5 antibody [EPR8236] (ab137014)
  • Anti-ENPP5 antibody [EPR8236] (ab137014)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8236] to ENPP5
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Recombinant Human ENPP5 protein (ab127853)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ENPP5 antibody [EPR8236]
    See all ENPP5 primary antibodies
  • Description

    Rabbit monoclonal [EPR8236] to ENPP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENPP5 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Y79, SH-SY5Y, MCF7, U87-MG, C6, and Neuro-2a cell lysates; MCF7 cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Dissociation constant (KD)

    KD = 1.18 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8236
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Regulation

Associated products

  • Alternative Versions

    • Anti-ENPP5 antibody [EPR8236] - BSA and Azide free (ab248766)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
  • Recombinant Protein

    • Recombinant Human ENPP5 protein (ab127853)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity.
    • Sequence similarities

      Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Secreted. Membrane.
    • Target information above from: UniProt accession Q9UJA9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 59084 Human
      • Entrez Gene: 83965 Mouse
      • Entrez Gene: 316249 Rat
      • SwissProt: Q9UJA9 Human
      • SwissProt: Q9EQG7 Mouse
      • SwissProt: P84039 Rat
      • Unigene: 35198 Human
      • Unigene: 30145 Mouse
      • Unigene: 451536 Mouse
      • Unigene: 198838 Rat
      see all
    • Alternative names

      • E-NPP 5 antibody
      • E-NPP5 antibody
      • ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function) antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase 5 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 5 antibody
      • ENPP5 antibody
      • ENPP5_HUMAN antibody
      • NPP-5 antibody
      • UNQ550/PRO1107 antibody
      see all

    Images

    • Western blot - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Western blot - Anti-ENPP5 antibody [EPR8236] (ab137014)
      All lanes : Anti-ENPP5 antibody [EPR8236] (ab137014) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Immunocytochemistry/ Immunofluorescence - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Immunofluorescent analysis of MCF7 cells labelling ENPP5 with ab137014 at 1/250 dilution.
    • OI-RD Scanning - Anti-ENPP5 antibody [EPR8236] (ab137014)
      OI-RD Scanning - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-ENPP5 antibody [EPR8236] (ab137014)
      Anti-ENPP5 antibody [EPR8236] (ab137014)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab137014? Please let us know so that we can cite the reference in this datasheet.

    ab137014 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Do you have any data regarding the ab137014 cross-reactivity vs. ENPP-4,-6, and -7? This further info would be of great help to us.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 19 2015

    Answer


    I am sorry to confirm that the cross reactivity of the antibody ab137014 with ENPP-4,-6, and -7 has not specifically been tested in the laboratory.

    However, I can let you know the alignments of the immunogen with the sequences of these proteins (I've used human sequences)


    ENPP-4 23%,
    ENPP-6 15%,
    ENPP-7 30%

    These are low which indicates the antibody will probably not detect these.

    I am sorry we have no data to confirm an answer to your question on this occasion. However, I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Sam Washer

    Abcam Scientific Support

    Answered on Jan 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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