Overview

  • Product name
    Anti-ENPP5 antibody [EPR8236]
    See all ENPP5 primary antibodies
  • Description
    Rabbit monoclonal [EPR8236] to ENPP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENPP5 aa 400-500. The exact sequence is proprietary.

  • Positive control
    • Y79, SH-SY5Y, MCF7, U87-MG, C6, and Neuro-2a cell lysates; MCF7 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Dissociation constant (KD)
    KD = 1.18 x 10 -11 M
    Learn more about KD
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8236
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-ENPP5 antibody [EPR8236] (ab137014) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    • Immunofluorescent analysis of MCF7 cells labelling ENPP5 with ab137014 at 1/250 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab137014 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer


    I am sorry to confirm that the cross reactivity of the antibody ab137014 with ENPP-4,-6, and -7 has not specifically been tested in the laboratory.

    However, I can let you know the alignments of the immunogen with the sequences of these proteins (I've used human sequences)


    ENPP-4 23%,
    ENPP-6 15%,
    ENPP-7 30%

    These are low which indicates the antibody will probably not detect these.

    I am sorry we have no data to confirm an answer to your question on this occasion. However, I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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