Recombinant Anti-ENPP5 antibody [EPR8236] (ab137014)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8236] to ENPP5
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ENPP5 antibody [EPR8236]
See all ENPP5 primary antibodies -
Description
Rabbit monoclonal [EPR8236] to ENPP5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ENPP5 aa 400-500. The exact sequence is proprietary.
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Positive control
- Y79, SH-SY5Y, MCF7, U87-MG, C6, and Neuro-2a cell lysates; MCF7 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 1.18 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8236 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Function
May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity. -
Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 59084 Human
- Entrez Gene: 83965 Mouse
- Entrez Gene: 316249 Rat
- SwissProt: Q9UJA9 Human
- SwissProt: Q9EQG7 Mouse
- SwissProt: P84039 Rat
- Unigene: 35198 Human
- Unigene: 30145 Mouse
see all -
Alternative names
- E-NPP 5 antibody
- E-NPP5 antibody
- ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function) antibody
see all
Images
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All lanes : Anti-ENPP5 antibody [EPR8236] (ab137014) at 1/1000 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
Immunofluorescent analysis of MCF7 cells labelling ENPP5 with ab137014 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137014 has not yet been referenced specifically in any publications.