Recombinant
RabMAb

Anti-ENPP5 antibody [EPR8236] (ab137014)

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Overview

  • Product name
    Anti-ENPP5 antibody [EPR8236]
    See all ENPP5 primary antibodies
  • Description
    Rabbit monoclonal [EPR8236] to ENPP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENPP5 aa 400-500. The exact sequence is proprietary.

  • Positive control
    • Y79, SH-SY5Y, MCF7, U87-MG, C6, and Neuro-2a cell lysates; MCF7 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Dissociation constant (KD)
    KD = 1.18 x 10 -11 M
    Learn more about KD
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8236
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Western blot - Anti-ENPP5 antibody [EPR8236] (ab137014)
      All lanes : Anti-ENPP5 antibody [EPR8236] (ab137014) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Immunocytochemistry/ Immunofluorescence - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Immunofluorescent analysis of MCF7 cells labelling ENPP5 with ab137014 at 1/250 dilution.
    • Other - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Other - Anti-ENPP5 antibody [EPR8236] (ab137014)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab137014 has not yet been referenced specifically in any publications.

    Publishing research using ab137014? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Do you have any data regarding the ab137014 cross-reactivity vs. ENPP-4,-6, and -7? This further info would be of great help to us.

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    Answer


    I am sorry to confirm that the cross reactivity of the antibody ab137014 with ENPP-4,-6, and -7 has not specifically been tested in the laboratory.

    However, I can let you know the alignments of the immunogen with the sequences of these proteins (I've used human sequences)


    ENPP-4 23%,
    ENPP-6 15%,
    ENPP-7 30%

    These are low which indicates the antibody will probably not detect these.

    I am sorry we have no data to confirm an answer to your question on this occasion. However, I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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