Overview

  • Product name

  • Description

    Rabbit polyclonal to ENPP6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 15-207 of Human ENPP6 (Q6UWR7).

  • Positive control

    • MCF7 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.

Target

  • Relevance

    ENPP6 is a choline-specific glycerophosphodiester phosphodiesterase, which hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5 prime-monophosphate. It hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. ENPP6 has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. ENPP6 also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. ENPP6 is predominantly expressed in kidney and brain.
  • Cellular localization

    Cell membrane; Single-pass type I membrane protein. Secreted. Note: A minor secreted form also exists.
  • Database links

  • Alternative names

    • B830047L21Rik antibody
    • E-NPP 6 antibody
    • Ectonucleotide pyrophosphatase/phosphodiesterase 6 antibody
    • ectonucleotide pyrophosphatase/phosphodiesterase family member 6 antibody
    • ENPP 6 antibody
    • NPP 6 antibody
    • NPP6 antibody
    see all

Images

  • Anti-ENPP6 antibody (ab127897) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS Page

References

This product has been referenced in:

  • Aranmolate A  et al. Myelination is delayed during postnatal brain development in the mdx mouse model of Duchenne muscular dystrophy. BMC Neurosci 18:63 (2017). Read more (PubMed: 28806929) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: RT°C
Fixative
Paraformaldehyde

Mr. Lin Xiao

Verified customer

Submitted Apr 13 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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