Anti-ENSA antibody (ab125873)
Key features and details
- Rabbit polyclonal to ENSA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ENSA antibody
See all ENSA primary antibodies -
Description
Rabbit polyclonal to ENSA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-102 of Human ENSA (NP_004427).
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Positive control
- A549 whole cell lysate HeLa whole cell lysate HCT116 whole cell lysate HeLa cells (methanol fixed)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 13 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 13 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents. -
Tissue specificity
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. -
Sequence similarities
Belongs to the endosulfine family. -
Post-translational
modificationsPhosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2029 Human
- Omim: 603061 Human
- SwissProt: O43768 Human
- Unigene: 632456 Human
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Alternative names
- Alpha endosulfine antibody
- Alpha-endosulfine antibody
- ARPP 19e antibody
see all
Images
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All lanes : Anti-ENSA antibody (ab125873) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : ENSA Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 13 kDa -
Anti-ENSA antibody (ab125873) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 13 kDa
15% SDS PAGE -
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ENSA (green) using ab125873 at 1/500 dilution in ICC/IF. Blue: Hoechst 33343 staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125873 has not yet been referenced specifically in any publications.