Overview

  • Product name

  • Description

    Rabbit polyclonal to ENSA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-102 of Human ENSA (NP_004427).

  • Positive control

    • A549 whole cell lysate HeLa whole cell lysate HCT116 whole cell lysate HeLa cells (methanol fixed)

Properties

Applications

Our Abpromise guarantee covers the use of ab125873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 13 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    ENSA (Alpha endosulfine) is expressed in a wide range of tissues including muscle, brain, and endocrine tissues. The recombinant protein inhibits binding of labeled glibenclamide to beta cell membranes. It also inhibits cloned K(ATP) channel currents and thereby stimulates insulin secretion. It was proposed that endosulfine is an endogenous regulator of the K(ATP) channel, which has a key role in the control of insulin release and, more generally, couples cell metabolism to electrical activity.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Alpha endosulfine antibody
    • ARPP 19e antibody
    • Endosulfine alpha antibody

Images

  • All lanes : Anti-ENSA antibody (ab125873) at 1/500 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : ENSA Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 13 kDa

  • Anti-ENSA antibody (ab125873) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 13 kDa



    15% SDS PAGE
  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ENSA (green) using ab125873 at 1/500 dilution in ICC/IF. Blue: Hoechst 33343 staining.

References

ab125873 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab125873.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up