Anti-ENSA antibody (ab247103)
Key features and details
- Rabbit polyclonal to ENSA
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ENSA antibody
See all ENSA primary antibodies -
Description
Rabbit polyclonal to ENSA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human ENSA aa 1-36.
Sequence:MSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAK
Database link: O43768 -
Positive control
- IHC-P: Human esophagus tissue. ICC/IF: PC-3 cells. WB: U-251 MG whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab247103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Relevance
ENSA (Alpha endosulfine) is expressed in a wide range of tissues including muscle, brain, and endocrine tissues. The recombinant protein inhibits binding of labeled glibenclamide to beta cell membranes. It also inhibits cloned K(ATP) channel currents and thereby stimulates insulin secretion. It was proposed that endosulfine is an endogenous regulator of the K(ATP) channel, which has a key role in the control of insulin release and, more generally, couples cell metabolism to electrical activity. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 281142 Cow
- Entrez Gene: 2029 Human
- Entrez Gene: 60334 Rat
- Omim: 603061 Human
- SwissProt: O43768 Human
- SwissProt: P60841 Rat
- Unigene: 632456 Human
- Unigene: 27154 Rat
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Alternative names
- Alpha endosulfine antibody
- ARPP 19e antibody
- Endosulfine alpha antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ENSA antibody (ab247103)
PFA-fixed, Triton X-100 permeabilized PC-3 cells stained for ENSA (green) using ab247103 at 4 μg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENSA antibody (ab247103)Paraffin-embedded human esophagus tissue stained for ENSA using ab247103 at 1/200 dilution in immunohistochemical analysis. -
Western blot - Anti-ENSA antibody (ab247103)All lanes : Anti-ENSA antibody (ab247103) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 13 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab247103 has not yet been referenced specifically in any publications.
