Anti-ENSA antibody (ab247103)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ENSA antibody
See all ENSA primary antibodies -
Description
Rabbit polyclonal to ENSA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human ENSA aa 1-36.
Sequence:MSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAK
Database link: O43768 -
Positive control
- IHC-P: Human esophagus tissue. ICC/IF: PC-3 cells. WB: U-251 MG whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab247103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.4 µg/ml. Predicted molecular weight: 13 kDa. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Relevance
ENSA (Alpha endosulfine) is expressed in a wide range of tissues including muscle, brain, and endocrine tissues. The recombinant protein inhibits binding of labeled glibenclamide to beta cell membranes. It also inhibits cloned K(ATP) channel currents and thereby stimulates insulin secretion. It was proposed that endosulfine is an endogenous regulator of the K(ATP) channel, which has a key role in the control of insulin release and, more generally, couples cell metabolism to electrical activity. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 281142 Cow
- Entrez Gene: 2029 Human
- Entrez Gene: 60334 Rat
- Omim: 603061 Human
- SwissProt: O43768 Human
- SwissProt: P60841 Rat
- Unigene: 632456 Human
- Unigene: 27154 Rat
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Alternative names
- Alpha endosulfine antibody
- ARPP 19e antibody
- Endosulfine alpha antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ENSA antibody (ab247103)PFA-fixed, Triton X-100 permeabilized PC-3 cells stained for ENSA (green) using ab247103 at 4 μg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENSA antibody (ab247103)Paraffin-embedded human esophagus tissue stained for ENSA using ab247103 at 1/200 dilution in immunohistochemical analysis. -
Western blot - Anti-ENSA antibody (ab247103)All lanes : Anti-ENSA antibody (ab247103) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 13 kDa
Datasheets and documents
References
ab247103 has not yet been referenced specifically in any publications.
