Recombinant
RabMAb

Recombinant Anti-ENSA antibody [EPR8008(2)] - BSA and Azide free (ab238993)

Overview

  • Product name

    Anti-ENSA antibody [EPR8008(2)] - BSA and Azide free
    See all ENSA primary antibodies
  • Description

    Rabbit monoclonal [EPR8008(2)] to ENSA - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ENSA (O43768) and ARPP-19 (P56211)
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENSA aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: O43768-2

  • Positive control

    • FC: K562 cells.
  • General notes

    Ab238993 is the carrier-free version of ab180513. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238993 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    ENSA (Alpha endosulfine) is expressed in a wide range of tissues including muscle, brain, and endocrine tissues. The recombinant protein inhibits binding of labeled glibenclamide to beta cell membranes. It also inhibits cloned K(ATP) channel currents and thereby stimulates insulin secretion. It was proposed that endosulfine is an endogenous regulator of the K(ATP) channel, which has a key role in the control of insulin release and, more generally, couples cell metabolism to electrical activity.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Alpha endosulfine antibody
    • ARPP 19e antibody
    • Endosulfine alpha antibody

Images

  • Flow cytometric analysis of K562 cells labeling ENSA with ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab180513).

References

ab238993 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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