Overview

  • Product name

  • Description

    Rabbit polyclonal to ENT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ENT1 aa 234-263 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control

    • Mouse lung, HL-60. This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135756 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
  • Tissue specificity

    Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
  • Sequence similarities

    Belongs to the SLC29A transporter family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Equilibrative NBMPR-sensitive nucleoside transporter antibody
    • equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 1 antibody
    • es-type antibody
    • MGC1465 antibody
    • MGC3778 antibody
    • Nucleoside transporter antibody
    • Nucleoside transporter, es-type antibody
    • OTTHUMP00000016506 antibody
    • OTTHUMP00000016507 antibody
    • OTTHUMP00000016508 antibody
    • OTTHUMP00000016509 antibody
    • OTTHUMP00000016510 antibody
    • OTTHUMP00000016511 antibody
    • OTTHUMP00000016512 antibody
    • S29A1_HUMAN antibody
    • Slc29a1 antibody
    • solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
    • solute carrier family 29 (nucleoside transporters), member 1 antibody
    • Solute carrier family 29 member 1 antibody
    see all

Images

  • Anti-ENT1 antibody (ab135756) at 1/1000 dilution + HL-60 cell line at 35 µg

    Secondary
    Goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size: 50 kDa

  • Anti-ENT1 antibody (ab135756) at 1/2000 dilution + mouse lung at 20 µg

    Secondary
    Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

     

  • ab135756 staining ENT1 in Human colorectal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween 20 and blocked with Protein Block Serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in 0.1M Sodium Citrate (82mls 0.1M) + Citric Acid (18mls 0.1 M) + Elga water (pH6). Samples were incubated with primary antibody (1/500 in Antibody Diluent) for 20 hours at 20°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab135756 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135756 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Ludwig KF  et al. Small-Molecule Inhibition of Axl Targets Tumor Immune Suppression and Enhances Chemotherapy in Pancreatic Cancer. Cancer Res 78:246-255 (2018). Read more (PubMed: 29180468) »
  • Koh SB  et al. Mechanistic Distinctions between CHK1 and WEE1 Inhibition Guide the Scheduling of Triple Therapy with Gemcitabine. Cancer Res 78:3054-3066 (2018). Read more (PubMed: 29735549) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, proximal tubule)
Permeabilization
Yes - 1% triton
Specification
kidney, proximal tubule
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

Submitted Mar 04 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Protein Block Serum Free X0909 as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate (82mls 0.1M) + Citric Acid (18mls 0.1 M) + Elga water (pH6)
Sample
Human Tissue sections (Colorectal cancer tissues)
Specification
Colorectal cancer tissues
Permeabilization
Yes - Tween 20
Fixative
Formaldehyde

Mr. Haoyu Wang

Verified customer

Submitted Mar 13 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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