Anti-ENT1 antibody (ab150493)
Key features and details
- Rabbit polyclonal to ENT1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ENT1 antibody
See all ENT1 primary antibodies -
Description
Rabbit polyclonal to ENT1 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Goat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Monkey, Gorilla, Common marmoset, Orangutan, Bat, Elephant
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Immunogen
Synthetic 16 amino acid peptide from the internal region of Human ENT1.
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Positive control
- Human placenta tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). -
Tissue specificity
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen. -
Sequence similarities
Belongs to the SLC29A transporter family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells. - Information by UniProt
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Database links
- Entrez Gene: 2030 Human
- Omim: 602193 Human
- SwissProt: Q99808 Human
- Unigene: 25450 Human
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Alternative names
- Equilibrative NBMPR-sensitive nucleoside transporter antibody
- equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
- Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody (ab150493)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labelling ENT1 with ab150493 at 2.5 µg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab150493 has not yet been referenced specifically in any publications.