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    ent1-antibody-ab150493.pdf

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Signal Transduction Metabolism Plasma Membrane Channels
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Anti-ENT1 antibody (ab150493)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody (ab150493)

    Key features and details

    • Rabbit polyclonal to ENT1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-ENT1 antibody
      See all ENT1 primary antibodies
    • Description

      Rabbit polyclonal to ENT1
    • Host species

      Rabbit
    • Specificity

      BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rabbit, Goat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Monkey, Gorilla, Common marmoset, Orangutan, Bat, Elephant
    • Immunogen

      Synthetic 16 amino acid peptide from the internal region of Human ENT1.

    • Positive control

      • Human placenta tissue
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab150493 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Target

    • Function

      Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
    • Tissue specificity

      Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
    • Sequence similarities

      Belongs to the SLC29A transporter family.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
    • Target information above from: UniProt accession Q99808 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2030 Human
      • Omim: 602193 Human
      • SwissProt: Q99808 Human
      • Unigene: 25450 Human
      • Alternative names

        • Equilibrative NBMPR-sensitive nucleoside transporter antibody
        • equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
        • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
        • Equilibrative nucleoside transporter 1 antibody
        • es-type antibody
        • MGC1465 antibody
        • MGC3778 antibody
        • Nucleoside transporter antibody
        • Nucleoside transporter, es-type antibody
        • OTTHUMP00000016506 antibody
        • OTTHUMP00000016507 antibody
        • OTTHUMP00000016508 antibody
        • OTTHUMP00000016509 antibody
        • OTTHUMP00000016510 antibody
        • OTTHUMP00000016511 antibody
        • OTTHUMP00000016512 antibody
        • S29A1_HUMAN antibody
        • Slc29a1 antibody
        • solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
        • solute carrier family 29 (nucleoside transporters), member 1 antibody
        • Solute carrier family 29 member 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody (ab150493)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody (ab150493)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labelling ENT1 with ab150493 at 2.5 µg/ml.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab150493? Please let us know so that we can cite the reference in this datasheet.

      ab150493 has not yet been referenced specifically in any publications.

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