Overview

  • Product name

    Anti-ENT1 antibody - N-terminal
    See all ENT1 primary antibodies
  • Description

    Goat polyclonal to ENT1 - N-terminal
  • Host species

    Goat
  • Specificity

    Reported variants represent identical protein: NP_001071642.1, NP_001071645.1, NP_001071644.1, NP_004946.1, NP_001071643.1.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human ENT1 aa 3-15 (N terminal) (Cysteine residue). (NP_001071643.1).
    Sequence:

    TSHQPQDRYKAVW


    Database link: Q99808

  • Positive control

    • WB: Human breast, spleen and placenta tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab190877 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
  • Tissue specificity

    Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
  • Sequence similarities

    Belongs to the SLC29A transporter family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Equilibrative NBMPR-sensitive nucleoside transporter antibody
    • equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 1 antibody
    • es-type antibody
    • MGC1465 antibody
    • MGC3778 antibody
    • Nucleoside transporter antibody
    • Nucleoside transporter, es-type antibody
    • OTTHUMP00000016506 antibody
    • OTTHUMP00000016507 antibody
    • OTTHUMP00000016508 antibody
    • OTTHUMP00000016509 antibody
    • OTTHUMP00000016510 antibody
    • OTTHUMP00000016511 antibody
    • OTTHUMP00000016512 antibody
    • S29A1_HUMAN antibody
    • Slc29a1 antibody
    • solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
    • solute carrier family 29 (nucleoside transporters), member 1 antibody
    • Solute carrier family 29 member 1 antibody
    see all

Images

  • All lanes : Anti-ENT1 antibody - N-terminal (ab190877) at 1 µg/ml

    Lane 1 : Human breast tissue lysates
    Lane 2 : Human placenta tissue lysates
    Lane 3 : Human spleen tissue lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa



    Detected by chemiluminescence.

References

ab190877 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190877.
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