Anti-ENT1 antibody [SP120] (ab182023)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-ENT1 antibody [SP120]
See all ENT1 primary antibodies -
DescriptionRabbit monoclonal [SP120] to ENT1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human ENT1 aa 250-350 (internal sequence). The exact sequence is proprietary.
Database link: Q99808 -
Positive control
- Human Pancreas tissue, Human tonsil tissue
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General notes
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from TCS by protein A/G.
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ClonalityMonoclonal
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Clone numberSP120
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab182023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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FunctionMediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
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Tissue specificityExpressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
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Sequence similaritiesBelongs to the SLC29A transporter family.
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Post-translational
modificationsGlycosylated. -
Cellular localizationBasolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
- Information by UniProt
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Database links
- Entrez Gene: 2030 Human
- Omim: 602193 Human
- SwissProt: Q99808 Human
- Unigene: 25450 Human
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Alternative names
- Equilibrative NBMPR-sensitive nucleoside transporter antibody
- equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
- Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] (ab182023)](https://www.abcam.com/ps/products/182/ab182023/Images/ab182023-215559-Image1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] (ab182023)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue labeling ENT1 with ab182023.
Protocols
References
ab182023 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"