Recombinant Anti-ENT2 antibody [EPR11674] (ab181192)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)](https://www.abcam.com/ps/products/181/ab181192/Images/ab181192-214665-anti-ent2-antibody-epr11674-immunohistochemistry.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11674] to ENT2
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ENT2 antibody [EPR11674]
See all ENT2 primary antibodies -
Description
Rabbit monoclonal [EPR11674] to ENT2 -
Host species
Rabbit -
Specificity
May be unsuitable with mouse in WB. -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HepG2, K562, Jurkat, SH-SY5Y cell lysates. Human RNT2 recombinant protein. Human clear cell carcinoma of the kidney tissue. HepG2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11674 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
1/100 - 1/250.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/100 - 1/250. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa). |
Target
-
Function
Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine. -
Tissue specificity
Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues. -
Sequence similarities
Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. -
Cellular localization
Basolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells. - Information by UniProt
-
Database links
- Entrez Gene: 3177 Human
- Entrez Gene: 13340 Mouse
- Entrez Gene: 100911721 Rat
- Entrez Gene: 65194 Rat
- Omim: 602110 Human
- SwissProt: Q14542 Human
- SwissProt: Q61672 Mouse
- SwissProt: Q9JIT8 Mouse
see all -
Alternative names
- 36 kDa antibody
- 36 kDa nucleolar protein HNP36 antibody
- Delayed-early response protein 12 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)](https://www.abcam.com/ps/products/181/ab181192/Images/ab181192-214663-anti-ent2-antibody-epr11674-western-blot.png)
Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)All lanes : Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution
Lane 1 : HepG2
Lane 2 : K562
Lane 3 : Jurkat
Lane 4 : SH-SY5Y
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 50 kDa
Additional bands at: 55-60 kDa (possible glycosylated form) -
![Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)](https://www.abcam.com/ps/products/181/ab181192/Images/ab181192-214666-anti-ent2-antibody-epr11674-immunofluorescence.png)
Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green). Counterstained with DAPI (blue).
-
![Flow Cytometry (Intracellular) - Anti-ENT2 antibody [EPR11674] (ab181192)](https://www.abcam.com/ps/products/181/ab181192/Images/ab181192-214667-anti-ent2-antibody-epr11674-flow-cytometry.png)
Flow Cytometry (Intracellular) - Anti-ENT2 antibody [EPR11674] (ab181192)Intracellular flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink). Rabbit monoclonal IgG was used as isotype control.
-
![Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)](https://www.abcam.com/ps/products/181/ab181192/Images/ab181192-214664-anti-ent2-antibody-epr11674-western-blot.png)
Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution + Human ENT2 recombinant protein, 10 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa -
![Anti-ENT2 antibody [EPR11674] (ab181192)](https://www.abcam.com/ps/products/181/ab181192/Images/ab181192-6-benefits-of-recombinant-antibodies.png)
Anti-ENT2 antibody [EPR11674] (ab181192)
Protocols
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (4)
ab181192 has been referenced in 4 publications.
- Liu P et al. e5NT inhibitor protects acute restraint stress-induced depression by regulating nucleoside release in mice. J Pharm Pharmacol 72:1556-1563 (2020). PubMed: 32743802
- Gandhi R et al. Cell proliferation detected using [18F]FLT PET/CT as an early marker of abdominal aortic aneurysm. J Nucl Cardiol N/A:N/A (2019). PubMed: 31741324
- Alarcón S et al. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy. Sci Rep 7:9439 (2017). PubMed: 28842605
- García CP et al. Topoisomerase I inhibitor, camptothecin, induces apoptogenic signaling in human embryonic stem cells. Stem Cell Res 12:400-14 (2014). PubMed: 24380814
