Product nameAnti-ENT2 antibody [EPR11674]
See all ENT2 primary antibodies
DescriptionRabbit monoclonal [EPR11674] to ENT2
SpecificityMay be unsuitable with mouse in WB.
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ENT2 aa 1-100. The exact sequence is proprietary.
Database link: Q14542
- HepG2, K562, Jurkat, SH-SY5Y cell lysates. Human RNT2 recombinant protein. Human clear cell carcinoma of the kidney tissue. HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).|
FunctionMediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.
Tissue specificityExpressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.
Sequence similaritiesBelongs to the SLC29A/ENT transporter (TC 2.A.57) family.
Cellular localizationBasolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells.
- Information by UniProt
- 36 kDa antibody
- 36 kDa nucleolar protein HNP36 antibody
- Delayed-early response protein 12 antibody
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
All lanes : Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution
Lane 1 : HepG2
Lane 2 : K562
Lane 3 : Jurkat
Lane 4 : SH-SY5Y
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 50 kDa
Additional bands at: 55-60 kDa (possible glycosylated form)
Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green). Counterstained with DAPI (blue).
Flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink). Rabbit monoclonal IgG was used as isotype control.
Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution + Human ENT2 recombinant protein, 10 ng
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
This product has been referenced in:
- Alarcón S et al. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy. Sci Rep 7:9439 (2017). Read more (PubMed: 28842605) »
- García CP et al. Topoisomerase I inhibitor, camptothecin, induces apoptogenic signaling in human embryonic stem cells. Stem Cell Res 12:400-14 (2014). Read more (PubMed: 24380814) »