Recombinant
RabMAb

Recombinant Anti-ENT2 antibody [EPR11674] (ab181192)

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Overview

  • Product name

    Anti-ENT2 antibody [EPR11674]
    See all ENT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11674] to ENT2

  • Host species

    Rabbit

  • Specificity

    May be unsuitable with mouse in WB.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ENT2 aa 1-100. The exact sequence is proprietary.
    Database link: Q14542

  • Positive control

    • HepG2, K562, Jurkat, SH-SY5Y cell lysates. Human RNT2 recombinant protein. Human clear cell carcinoma of the kidney tissue. HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11674

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.

  • Tissue specificity

    Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.

  • Sequence similarities

    Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

  • Cellular localization

    Basolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 36 kDa antibody
    • 36 kDa nucleolar protein HNP36 antibody
    • Delayed-early response protein 12 antibody
    • DER12 antibody
    • ei-type antibody
    • Equilibrative NBMPR-insensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 2 antibody
    • HNP36 antibody
    • Hydrophobic nucleolar protein antibody
    • Hydrophobic nucleolar protein, 36 kDa antibody
    • Nucleoside transporter antibody
    • S29A2_HUMAN antibody
    • SLC29A2 antibody
    • solute carrier family 29 (nucleoside transporters), member 29 antibody
    • Solute carrier family 29 member 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)

    Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    All lanes : Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution

    Lane 1 : HepG2
    Lane 2 : K562
    Lane 3 : Jurkat
    Lane 4 : SH-SY5Y

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 50 kDa
    Additional bands at: 55-60 kDa (possible glycosylated form)

  • Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)
    Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)

    Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde  labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green).  Counterstained with DAPI (blue).

  • Flow Cytometry - Anti-ENT2 antibody [EPR11674] (ab181192)
    Flow Cytometry - Anti-ENT2 antibody [EPR11674] (ab181192)

    Flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink).  Rabbit monoclonal IgG was used as isotype control.

  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution + Human ENT2 recombinant protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

References

This product has been referenced in:

  • Alarcón S  et al. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy. Sci Rep 7:9439 (2017). Read more (PubMed: 28842605) »
  • García CP  et al. Topoisomerase I inhibitor, camptothecin, induces apoptogenic signaling in human embryonic stem cells. Stem Cell Res 12:400-14 (2014). Read more (PubMed: 24380814) »
See all 2 Publications for this product

Publishing research using ab181192? Please let us know so that we can cite the reference in this datasheet.

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