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    ent2-antibody-epr11674-ab181192.pdf

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Signal Transduction Protein Trafficking Nuclear Import / Export
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RecombinantRabMAb

Recombinant Anti-ENT2 antibody [EPR11674] (ab181192)

  • Datasheet
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Flow Cytometry (Intracellular) - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Anti-ENT2 antibody [EPR11674] (ab181192)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11674] to ENT2
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Recombinant Human ENT2 protein (ab152449)
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Anti-SLC28A1 antibody (ab192438)

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Overview

  • Product name

    Anti-ENT2 antibody [EPR11674]
    See all ENT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11674] to ENT2
  • Host species

    Rabbit
  • Specificity

    May be unsuitable with mouse in WB.
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, K562, Jurkat, SH-SY5Y cell lysates. Human RNT2 recombinant protein. Human clear cell carcinoma of the kidney tissue. HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11674
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (ab201255)
    • Anti-ENT2 antibody [EPR11674] - BSA and Azide free (ab231685)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human ENT2 protein (ab152449)
  • Related Products

    • Recombinant Human ENT2 protein (ab152449)
    • Jurkat whole cell lysate (ab7899)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).
Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.
  • Tissue specificity

    Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.
  • Sequence similarities

    Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
  • Cellular localization

    Basolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells.
  • Target information above from: UniProt accession Q14542 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3177 Human
    • Entrez Gene: 13340 Mouse
    • Entrez Gene: 100911721 Rat
    • Entrez Gene: 65194 Rat
    • Omim: 602110 Human
    • SwissProt: Q14542 Human
    • SwissProt: Q61672 Mouse
    • SwissProt: Q9JIT8 Mouse
    • SwissProt: O54699 Rat
    • Unigene: 569017 Human
    • Unigene: 4930 Mouse
    • Unigene: 37517 Rat
    see all
  • Alternative names

    • 36 kDa antibody
    • 36 kDa nucleolar protein HNP36 antibody
    • Delayed-early response protein 12 antibody
    • DER12 antibody
    • ei-type antibody
    • Equilibrative NBMPR-insensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 2 antibody
    • HNP36 antibody
    • Hydrophobic nucleolar protein antibody
    • Hydrophobic nucleolar protein, 36 kDa antibody
    • Nucleoside transporter antibody
    • S29A2_HUMAN antibody
    • SLC29A2 antibody
    • solute carrier family 29 (nucleoside transporters), member 29 antibody
    • Solute carrier family 29 member 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)

    Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    All lanes : Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution

    Lane 1 : HepG2
    Lane 2 : K562
    Lane 3 : Jurkat
    Lane 4 : SH-SY5Y

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 50 kDa
    Additional bands at: 55-60 kDa (possible glycosylated form)

  • Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)
    Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)

    Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde  labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green).  Counterstained with DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-ENT2 antibody [EPR11674] (ab181192)
    Flow Cytometry (Intracellular) - Anti-ENT2 antibody [EPR11674] (ab181192)

    Intracellular flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink). Rabbit monoclonal IgG was used as isotype control. 

     

     

  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution + Human ENT2 recombinant protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Anti-ENT2 antibody [EPR11674] (ab181192)
    Anti-ENT2 antibody [EPR11674] (ab181192)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (4)

Publishing research using ab181192? Please let us know so that we can cite the reference in this datasheet.

ab181192 has been referenced in 4 publications.

  • Liu P  et al. e5NT inhibitor protects acute restraint stress-induced depression by regulating nucleoside release in mice. J Pharm Pharmacol 72:1556-1563 (2020). PubMed: 32743802
  • Gandhi R  et al. Cell proliferation detected using [18F]FLT PET/CT as an early marker of abdominal aortic aneurysm. J Nucl Cardiol N/A:N/A (2019). PubMed: 31741324
  • Alarcón S  et al. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy. Sci Rep 7:9439 (2017). PubMed: 28842605
  • García CP  et al. Topoisomerase I inhibitor, camptothecin, induces apoptogenic signaling in human embryonic stem cells. Stem Cell Res 12:400-14 (2014). PubMed: 24380814

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