RecombinantRabMAb

Recombinant Anti-ENT2 antibody [EPR11674] (ab181192)

Submit a review Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Flow Cytometry - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
  • Anti-ENT2 antibody [EPR11674] (ab181192)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11674] to ENT2
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ENT2 antibody [EPR11674]
    See all ENT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11674] to ENT2

  • Host species

    Rabbit

  • Specificity

    May be unsuitable with mouse in WB.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, K562, Jurkat, SH-SY5Y cell lysates. Human RNT2 recombinant protein. Human clear cell carcinoma of the kidney tissue. HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11674

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).
Notes
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/2000. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.

  • Tissue specificity

    Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.

  • Sequence similarities

    Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

  • Cellular localization

    Basolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 36 kDa antibody
    • 36 kDa nucleolar protein HNP36 antibody
    • Delayed-early response protein 12 antibody
    • DER12 antibody
    • ei-type antibody
    • Equilibrative NBMPR-insensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 2 antibody
    • HNP36 antibody
    • Hydrophobic nucleolar protein antibody
    • Hydrophobic nucleolar protein, 36 kDa antibody
    • Nucleoside transporter antibody
    • S29A2_HUMAN antibody
    • SLC29A2 antibody
    • solute carrier family 29 (nucleoside transporters), member 29 antibody
    • Solute carrier family 29 member 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT2 antibody [EPR11674] (ab181192)

    Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    All lanes : Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution

    Lane 1 : HepG2
    Lane 2 : K562
    Lane 3 : Jurkat
    Lane 4 : SH-SY5Y

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 50 kDa
    Additional bands at: 55-60 kDa (possible glycosylated form)

  • Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)
    Immunocytochemistry/ Immunofluorescence - Anti-ENT2 antibody [EPR11674] (ab181192)

    Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde  labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green).  Counterstained with DAPI (blue).

  • Flow Cytometry - Anti-ENT2 antibody [EPR11674] (ab181192)
    Flow Cytometry - Anti-ENT2 antibody [EPR11674] (ab181192)

    Flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink).  Rabbit monoclonal IgG was used as isotype control.

  • Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Western blot - Anti-ENT2 antibody [EPR11674] (ab181192)
    Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution + Human ENT2 recombinant protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Anti-ENT2 antibody [EPR11674] (ab181192)
    Anti-ENT2 antibody [EPR11674] (ab181192)

References (3)

Publishing research using ab181192? Please let us know so that we can cite the reference in this datasheet.

ab181192 has been referenced in 3 publications.

  • Gandhi R  et al. Cell proliferation detected using [18F]FLT PET/CT as an early marker of abdominal aortic aneurysm. J Nucl Cardiol N/A:N/A (2019). PubMed: 31741324
  • Alarcón S  et al. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy. Sci Rep 7:9439 (2017). PubMed: 28842605
  • García CP  et al. Topoisomerase I inhibitor, camptothecin, induces apoptogenic signaling in human embryonic stem cells. Stem Cell Res 12:400-14 (2014). PubMed: 24380814

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