Recombinant
RabMAb

Recombinant Anti-ENT2 antibody [EPR11674] - BSA and Azide free (ab231685)

Overview

  • Product name

    Anti-ENT2 antibody [EPR11674] - BSA and Azide free
    See all ENT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11674] to ENT2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENT2 aa 1-100. The exact sequence is proprietary.
    Database link: Q14542

  • General notes

    Ab231685 is the carrier-free version of ab181192. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab231685 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11674
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.
  • Tissue specificity

    Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.
  • Sequence similarities

    Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
  • Cellular localization

    Basolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 36 kDa antibody
    • 36 kDa nucleolar protein HNP36 antibody
    • Delayed-early response protein 12 antibody
    • DER12 antibody
    • ei-type antibody
    • Equilibrative NBMPR-insensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 2 antibody
    • HNP36 antibody
    • Hydrophobic nucleolar protein antibody
    • Hydrophobic nucleolar protein, 36 kDa antibody
    • Nucleoside transporter antibody
    • S29A2_HUMAN antibody
    • SLC29A2 antibody
    • solute carrier family 29 (nucleoside transporters), member 29 antibody
    • Solute carrier family 29 member 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181192).

  • Flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink).  Rabbit monoclonal IgG was used as isotype control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181192).

  • Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde  labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green).  Counterstained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181192).

References

ab231685 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab231685.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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