Recombinant Anti-ENT2 antibody [EPR11674] - BSA and Azide free (ab231685)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11674] to ENT2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ENT2 antibody [EPR11674] - BSA and Azide free
See all ENT2 primary antibodies -
Description
Rabbit monoclonal [EPR11674] to ENT2 - BSA and Azide free -
Host species
Rabbit -
Specificity
May be unsuitable with mouse in WB.
-
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab231685 is the carrier-free version of ab181192.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11674 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-ENT2 antibody [EPR11674] (ab181192)
- Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (ab201255)
- PE Anti-ENT2 antibody [EPR11674] (ab306361)
- APC Anti-ENT2 antibody [EPR11674] (ab306362)
- HRP Anti-ENT2 antibody [EPR11674] (ab306363)
- Alexa Fluor® 647 Anti-ENT2 antibody [EPR11674] (ab310137)
- Alexa Fluor® 594 Anti-ENT2 antibody [EPR11674] (ab310559)
- Alexa Fluor® 555 Anti-ENT2 antibody [EPR11674] (ab312088)
- Alexa Fluor® 568 Anti-ENT2 antibody [EPR11674] (ab312567)
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 55-60 kDa (predicted molecular weight: 50 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine. -
Tissue specificity
Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues. -
Sequence similarities
Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. -
Cellular localization
Basolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells. - Information by UniProt
-
Database links
- Entrez Gene: 3177 Human
- Entrez Gene: 13340 Mouse
- Entrez Gene: 100911721 Rat
- Entrez Gene: 65194 Rat
- Omim: 602110 Human
- SwissProt: Q14542 Human
- SwissProt: Q61672 Mouse
- SwissProt: Q9JIT8 Mouse
see all -
Alternative names
- 36 kDa antibody
- 36 kDa nucleolar protein HNP36 antibody
- Delayed-early response protein 12 antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181192).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Intracellular flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink). Rabbit monoclonal IgG was used as isotype control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181192).
-
Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green). Counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181192).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab231685 has not yet been referenced specifically in any publications.