Product nameAnti-Entactin / NID antibody
See all Entactin / NID primary antibodies
DescriptionRabbit polyclonal to Entactin / NID
Tested applicationsSuitable for: WB, IHC-Fr, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse Entactin/ NID.
Previously labelled as Entactin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Our Abpromise guarantee covers the use of ab14511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||1/200. PubMed: 23409002|
FunctionSulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Sequence similaritiesContains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain.
modificationsN- and O-glycosylated.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Enactin antibody
- Ent antibody
- Entactin 1 antibody
ab14511 staining Entactin / NID in rat heart endocardium by Immunohistochemistry (Frozen sections)Tissue was fixed in acetone and permeabilized using Tween 20. Samples were then blocked with 5% serum for 1 hour at room temperature and then incubated with ab14511 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an undiluted chicken anti-rabbit IgG conjugated to Alexa Fluor 647 (blue).
This product has been referenced in:
- Herrera C et al. Effects of PI and PIII Snake Venom Haemorrhagic Metalloproteinases on the Microvasculature: A Confocal Microscopy Study on the Mouse Cremaster Muscle. PLoS One 11:e0168643 (2016). IHC ; Mouse . Read more (PubMed: 27992592) »
- Herrera C et al. Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis. PLoS Negl Trop Dis 10:e0004599 (2016). Read more (PubMed: 27035343) »