Overview

  • Product name
    Anti-Entactin / NID antibody
    See all Entactin / NID primary antibodies
  • Description
    Rabbit polyclonal to Entactin / NID
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse Entactin/ NID.

  • General notes

    Previously labelled as Entactin.

Properties

Applications

Our Abpromise guarantee covers the use of ab14511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200. PubMed: 23409002

Target

  • Function
    Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
  • Sequence similarities
    Contains 6 EGF-like domains.
    Contains 4 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enactin antibody
    • Ent antibody
    • Entactin 1 antibody
    • Entactin antibody
    • NID 1 antibody
    • NID antibody
    • NID-1 antibody
    • Nid1 antibody
    • NID1_HUMAN antibody
    • Nidogen (enactin) antibody
    • Nidogen 1 antibody
    • Nidogen antibody
    • Nidogen-1 antibody
    see all

Images

  • ab14511 staining Entactin / NID in rat heart endocardium by Immunohistochemistry (Frozen sections)Tissue was fixed in acetone and permeabilized using Tween 20. Samples were then blocked with 5% serum for 1 hour at room temperature and then incubated with ab14511 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an undiluted chicken anti-rabbit IgG conjugated to Alexa Fluor 647 (blue).

    See Abreview

References

This product has been referenced in:
  • Herrera C  et al. Effects of PI and PIII Snake Venom Haemorrhagic Metalloproteinases on the Microvasculature: A Confocal Microscopy Study on the Mouse Cremaster Muscle. PLoS One 11:e0168643 (2016). IHC ; Mouse . Read more (PubMed: 27992592) »
  • Herrera C  et al. Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis. PLoS Negl Trop Dis 10:e0004599 (2016). Read more (PubMed: 27035343) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (retinal tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Heat mediated antigen retrieval solution pH: 6 - 250 ml abcam ref ab973)
Permeabilization
Yes - 0.1% Tween 20 during 5 min
Specification
retinal tissue
Blocking step
Protein Block serum-free DAKO x 0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Metanol 95% C/ Acético Glacial 5%

Dr. Patricia Bogdanov

Verified customer

Submitted Jul 18 2016

Answer

Thank you for your enquiry. I can confirm that both ab14511 and ab14513 are sold as whole antiserum. As antibody concentration is usually determined by a protein assay, I am sorry it is not possible to provide a concentration for antibody in antiserum as it contains many other proteins. As a general guideline, antiserum is thought to contain about 1 - 10 mg/ml of antibody. I am sorry we are not able to be more precise on this occasion. If you have any further questions, please do not hesitate to contact us.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Frozen heart endocardium, 5 micron thick)
Permeabilization
Yes - Tween-20
Specification
Frozen heart endocardium, 5 micron thick
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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