Overview

  • Product name
  • Description
    Rabbit polyclonal to Entactin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein (Mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab14511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200. PubMed: 23409002

Target

  • Function
    Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
  • Sequence similarities
    Contains 6 EGF-like domains.
    Contains 4 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enactin antibody
    • Ent antibody
    • Entactin 1 antibody
    • Entactin antibody
    • NID 1 antibody
    • NID antibody
    • NID-1 antibody
    • Nid1 antibody
    • NID1_HUMAN antibody
    • Nidogen (enactin) antibody
    • Nidogen 1 antibody
    • Nidogen antibody
    • Nidogen-1 antibody
    see all

Images

  • ab14511 staining Entactin in rat heart endocardium by Immunohistochemistry (Frozen sections)
    Tissue was fixed in acetone and permeabilized using Tween 20. Samples were then blocked with 5% serum for 1 hour at room temperature and then incubated with ab14511 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an undiluted chicken anti-rabbit IgG conjugated to Alexa Fluor 647 (blue).

    See Abreview

References

This product has been referenced in:
  • Herrera C  et al. Effects of PI and PIII Snake Venom Haemorrhagic Metalloproteinases on the Microvasculature: A Confocal Microscopy Study on the Mouse Cremaster Muscle. PLoS One 11:e0168643 (2016). IHC ; Mouse . Read more (PubMed: 27992592) »
  • Herrera C  et al. Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis. PLoS Negl Trop Dis 10:e0004599 (2016). Read more (PubMed: 27035343) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I can confirm that both ab14511 and ab14513 are sold as whole antiserum. As antibody concentration is usually determined by a protein assay, I am sorry it is not possible to provide a concentration for antibody in antiser...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up