Key features and details
- Rabbit polyclonal to Entactin/NID
- Suitable for: WB, IHC-Fr, ELISA
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Entactin/NID antibody
See all Entactin/NID primary antibodies
DescriptionRabbit polyclonal to Entactin/NID
Tested applicationsSuitable for: WB, IHC-Fr, ELISAmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse Entactin/NID.
Previously labelled as Entactin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
Our Abpromise guarantee covers the use of ab14511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
FunctionSulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Sequence similaritiesContains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain.
modificationsN- and O-glycosylated.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Enactin antibody
- Ent antibody
- Entactin 1 antibody
ab14511 staining Entactin/NID in rat heart endocardium by Immunohistochemistry (Frozen sections)Tissue was fixed in acetone and permeabilized using Tween 20. Samples were then blocked with 5% serum for 1 hour at room temperature and then incubated with ab14511 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an undiluted chicken anti-rabbit IgG conjugated to Alexa Fluor 647 (blue).
ab14511 has been referenced in 10 publications.
- Molè MA et al. Integrin-Mediated Focal Anchorage Drives Epithelial Zippering during Mouse Neural Tube Closure. Dev Cell 52:321-334.e6 (2020). PubMed: 32049039
- Carrara N et al. Temporal characterization of optic fissure basement membrane composition suggests nidogen may be an initial target of remodeling. Dev Biol 452:43-54 (2019). PubMed: 31034836
- Herrera C et al. Effects of PI and PIII Snake Venom Haemorrhagic Metalloproteinases on the Microvasculature: A Confocal Microscopy Study on the Mouse Cremaster Muscle. PLoS One 11:e0168643 (2016). IHC ; Mouse . PubMed: 27992592
- Herrera C et al. Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis. PLoS Negl Trop Dis 10:e0004599 (2016). PubMed: 27035343
- Murphy PA et al. Tumor angiogenesis in the absence of fibronectin or its cognate integrin receptors. PLoS One 10:e0120872 (2015). PubMed: 25807551
- Herrera C et al. Tissue localization and extracellular matrix degradation by PI, PII and PIII snake venom metalloproteinases: clues on the mechanisms of venom-induced hemorrhage. PLoS Negl Trop Dis 9:e0003731 (2015). PubMed: 25909592
- Longmate WM et al. Reduced Fibulin-2 Contributes to Loss of Basement Membrane Integrity and Skin Blistering in Mice Lacking Integrin a3ß1 in the Epidermis. J Invest Dermatol N/A:N/A (2014). Mouse . PubMed: 24390135
- Hummitzsch K et al. A new model of development of the mammalian ovary and follicles. PLoS One 8:e55578 (2013). ICC/IF ; Cow . PubMed: 23409002
- Danussi C et al. EMILIN1-a4/a9 integrin interaction inhibits dermal fibroblast and keratinocyte proliferation. J Cell Biol 195:131-45 (2011). IHC-Fr ; Mouse . PubMed: 21949412
- Escalante T et al. Role of collagens and perlecan in microvascular stability: exploring the mechanism of capillary vessel damage by snake venom metalloproteinases. PLoS One 6:e28017 (2011). WB ; Mouse . PubMed: 22174764