Overview

  • Product name

    Anti-Entactin/NID antibody [EPR8962(B)]
    See all Entactin/NID primary antibodies
  • Description

    Rabbit monoclonal [EPR8962(B)] to Entactin/NID
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Entactin/NID aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Human fetal heart and fetal lung lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Entactin.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
    • Sequence similarities

      Contains 6 EGF-like domains.
      Contains 4 LDL-receptor class B repeats.
      Contains 1 NIDO domain.
      Contains 1 nidogen G2 beta-barrel domain.
      Contains 1 thyroglobulin type-1 domain.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Enactin antibody
      • Ent antibody
      • Entactin 1 antibody
      • Entactin antibody
      • NID 1 antibody
      • NID antibody
      • NID-1 antibody
      • Nid1 antibody
      • NID1_HUMAN antibody
      • Nidogen (enactin) antibody
      • Nidogen 1 antibody
      • Nidogen antibody
      • Nidogen-1 antibody
      see all

    Images

    • All lanes : Anti-Entactin/NID antibody [EPR8962(B)] (ab133686) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 136 kDa

    References

    This product has been referenced in:

    • Aleckovic M  et al. Identification of Nidogen 1 as a lung metastasis protein through secretome analysis. Genes Dev 31:1439-1455 (2017). Read more (PubMed: 28827399) »
    • Zhu D  et al. Nidogen-1 is a common target of microRNAs MiR-192/215 in the pathogenesis of Hirschsprung's disease. J Neurochem 134:39-46 (2015). Read more (PubMed: 25857602) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133686.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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