Overview

  • Product name

    Anti-entB antibody [3D10]
    See all entB primary antibodies
  • Description

    Mouse monoclonal [3D10] to entB
  • Host species

    Mouse
  • Specificity

    ab190465 does not appear to cross react with TSST-1 or SEC-1.
  • Tested applications

    Suitable for: Neutralising, WB, Sandwich ELISAmore details
    Unsuitable for: ELISA
  • Species reactivity

    Reacts with: Staphylococcus aureus
  • Immunogen

    Full length protein corresponding to entB.
    Database link: P01552

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    3D10
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration.

10 nM of ab190465 completely inhibits superantigenic activity of 0.1 ng/ml SEB.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
Sandwich ELISA Use a concentration of 1 µg/ml.

ab190465 can be used for capture of SEB in an ELISA sandwich assay.

ab190337 can be used as detection antibody, along with another anti rabbit HRP antibody.

  • Application notes
    Is unsuitable for ELISA.
  • Target

    • Relevance

      Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. SEB is an extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of SEB.
    • Cellular localization

      Secreted
    • Database links

      • Alternative names

        • Ent B antibody
        • EntB antibody
        • Enterotoxin type B antibody
        • SEB antibody
        • Staphylococcal enterotoxin B antibody
        • Staphylococcus Enterotoxin B antibody
        see all

      Images

      • Toxin Neutralization: dose dependent suppression of entB-induced IFNγ production in Human peripheral mononuclear cells (PBMC).

      • Capture ELISA in which ab190465 was coated to the plate overnight at 4oC at a concentration of 1 μg/mL. entB was serially diluted semi-log (1:3.16 serial dilutions) from 20 ng/mL and incubated on the antibody coated plate. Captured entB was detected using an anti-entB polyclonal antibody and anti-rabbit conjugate HRP conjugate. Data represent three different curves detected across the plate.

      • Anti-entB antibody [3D10] (ab190465) at 1 µg/ml + entB at 0.05 µg

        Secondary
        anti-mouse HRP conjugate.

        Predicted band size: 31 kDa

      References

      ab190465 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      There are currently no Customer reviews or Questions for ab190465.
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