Key features and details
- Mouse monoclonal [3D10] to entB
- Suitable for: Neutralising, WB, Sandwich ELISA
- Reacts with: Staphylococcus aureus
- Isotype: IgG1
Product nameAnti-entB antibody [3D10]
See all entB primary antibodies
DescriptionMouse monoclonal [3D10] to entB
Specificityab190465 does not appear to cross react with TSST-1 or SEC-1.
Tested applicationsSuitable for: Neutralising, WB, Sandwich ELISAmore details
Unsuitable for: ELISA
Species reactivityReacts with: Staphylococcus aureus
Full length protein corresponding to entB.
Database link: P01552
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab190465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use at an assay dependent concentration.
10 nM of ab190465 completely inhibits superantigenic activity of 0.1 ng/ml SEB.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.|
|Sandwich ELISA||Use a concentration of 1 µg/ml.
ab190465 can be used for capture of SEB in an ELISA sandwich assay.
ab190337 can be used as detection antibody, along with another anti rabbit HRP antibody.
RelevanceStaphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. SEB is an extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of SEB.
- Ent B antibody
- EntB antibody
- Enterotoxin type B antibody
Toxin Neutralization: dose dependent suppression of entB-induced IFNγ production in Human peripheral mononuclear cells (PBMC).
Capture ELISA in which ab190465 was coated to the plate overnight at 4oC at a concentration of 1 μg/mL. entB was serially diluted semi-log (1:3.16 serial dilutions) from 20 ng/mL and incubated on the antibody coated plate. Captured entB was detected using an anti-entB polyclonal antibody and anti-rabbit conjugate HRP conjugate. Data represent three different curves detected across the plate.
Anti-entB antibody [3D10] (ab190465) at 1 µg/ml + entB at 0.05 µg
anti-mouse HRP conjugate.
Predicted band size: 31 kDa
ab190465 has not yet been referenced specifically in any publications.