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  1. Link

    entb-antibody-ab15898.pdf

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Microbiology Toxin Bacterial Toxin Staphylococcus Toxin
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Anti-entB antibody (ab15898)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (3)

Product price, shipping and contact information

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Key features and details

  • Rabbit polyclonal to entB
  • Suitable for: ELISA, WB
  • Reacts with: Staphylococcus aureus
  • Isotype: IgG

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Overview

  • Product name

    Anti-entB antibody
    See all entB primary antibodies
  • Description

    Rabbit polyclonal to entB
  • Host species

    Rabbit
  • Specificity

    Absorbance readings (410 nm) of less than 0.100 for 10 ng/ml preparations of staphylococcal enterotoxins A through E (excluding B), ET, TSST, and alpha hemolysin.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Staphylococcus aureus
  • Immunogen

    Full length native protein (purified) corresponding to entB.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Constituents: 0.0268% PBS, 0.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Toxin
    • Bacterial Toxin
    • Staphylococcus Toxin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
Notes
ELISA
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.

Target

  • Relevance

    Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. SEB is an extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of SEB.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 3237776 Staphylococcus aureus
    • SwissProt: P01552 Staphylococcus aureus
    • Alternative names

      • Ent B antibody
      • EntB antibody
      • Enterotoxin type B antibody
      • SEB antibody
      • Staphylococcal enterotoxin B antibody
      • Staphylococcus Enterotoxin B antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab15898? Please let us know so that we can cite the reference in this datasheet.

    ab15898 has been referenced in 3 publications.

    • Mota-Martorell N  et al. Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity. Redox Biol 34:101539 (2020). PubMed: 32353747
    • Soykut EA  et al. Selection of staphylococcal enterotoxin B (SEB)-binding peptide using phage display technology. Biochem Biophys Res Commun 370:104-8 (2008). PubMed: 18359289
    • Branen JR  et al. An enzymatic bionanotransduction system for multianalyte biological detection. J Appl Microbiol 102:892-908 (2007). PubMed: 17381732

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    My company (BBInternational) is looking to develop a lateral flow diagnostic test for Staphylococcus aureus enterotoxin B (SEB).

    Could you please supply me with a quotes for 2 mg of each of the following antibody products from you catalogue for our initial development trials, and for 50 mg of each in the event that one is selected for our final product manufacture.



    Product code: ab15898 Rabbit polyclonal

    Product code: ab15911 Rabbit polyclonal (biotin)

    Product code: ab15919 sheep polyclonal

    Product code: ab15929 sheep polyclonal (biotin)

    Product code: ab6064 Mouse monoclonal

    Product code: ab36471 mouse monoclonal

    Product code: ab686 mouse monoclonal



    Also, do you have any information regarding which of the available antibodies functionally perform the best?

    Read More

    Abcam community

    Verified customer

    Asked on May 01 2012

    Answer

    Thank you for contacting us.

    I am sorry to inform you, but please be aware that our products are for research only and not suitable to be used as diagnostic tools. This information can be found in our Terms and Conditions under Product use limitations:


    "All Abcam products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC, THERAPEUTIC OR ANY IN VIVO USE"

    Abcam will not be liable for any professional advice it may offer in relation to the use of the products nor any claims or applications not listed in the literature or the misuse of products which will include using the products for diagnostic, therapeutic or in vivo use in human subjects. "

    I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on May 01 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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