Overview

  • Product name
    Anti-Enterococcus antibody
    See all Enterococcus primary antibodies
  • Description
    Rabbit polyclonal to Enterococcus
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with
  • Immunogen

    Enterococcus faecium.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS

    No stabilizing proteins have been added.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >95% pure.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notes
    ICC/IF: 1/1000.
    May be used in place of neat antiserum in almost any appropriate antibody based technique. Also suitable for conjugation purposes.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Enterococci form part of the lactic acid bacteria family and are the third most important bacterial genus in hospital infections, especially Enterococcus (E.) faecium. In food they can spoil processed meats but they are equally important for ripening and aroma development of certain traditional cheeses and sausages, and are used as human probiotics. However, they possesses a broad spectrum antibiotic resistance which has important implications to human health and disease.
    • Alternative names
      • E. faecium antibody
      • E.faecalis antibody

    Images

    • Immunohistochemical analysis of porcine plasma-based in vitro grown biofilm, staining Enterococcus with ab19980.

      Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes a room temperature. Samples were incubated with primary antibody (1/10000 in blocking buffer) for 18 hours at 4°C. Staining was detected using DAB.

      See Abreview

    References

    ab19980 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-10 of 10 Abreviews or Q&A

    Answer


    This antibody will detect the whole cells, and because the antiserum has not been absorbed (only Protein A purified) it may react with related microorganisms.


    As this antibody is suitable for conjugation purpose according to the datasheet, I would like to sduggest your customer is having a look at our FITC conjugation kit.


    Read More

    Answer

    Thank you for contacting us.

    I have attached the list of antibodies we have along with details. Please check.

    I am sorry I am unable to confirm the species cross reactivity of the these antibodies other than waht is given on the datasheet.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Answer

    Thank you for contacting us.

    ab41181 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

    ab41203 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

    ab19980 is an anti Enterococcus antibody

    ab68450, ab69185 and ab68451 are anti E. Coli antibodies.

    ab69468, ab20947, ab69467 and ab65926 are the Klebsiella spp specific antibodies.

    We do not have anti Strep. uberis antibodies.

    Please check our new western blot range:

    https://www.abcam.com/index.html?c=5634

    https://www.abcam.com/index.html?pageconfig=productmap&cl=918

    https://www.abcam.com/index.html?pageconfig=resource&rid=12886

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Answer

    Thank you for your enquiry.

    This is to confirm that the status of this Abreview has been changed from edited to submitted. It will be processed shortly by a member of our Scientific Team.

    If you need any further assistance in the future, please do not hesitate to contact me.

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    Answer

    Thank you for your response.

    This is to confirm that the two other Abreviews have been published and the Discount codes are active. As soon as the third Abreview is submitted, its Discount code will be activated as well.

    Good luck with the experiments and I am looking forward to receiving the Abreview.

    If you need any further assistance in the future, please do not hesitate to contact me.

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (porcine plasma-based in vitro grown biofilm)
    Specification
    porcine plasma-based in vitro grown biofilm
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

    Jan Kucera

    Verified customer

    Submitted Nov 08 2012

    Answer

    Thank you for your enquiry.

    I have changed the status from edited to submitted of these two Abreviews (ab19980 and ab20556). They have come through to our internal system without any problem so they will be processed very shortly by one of the members of our Scientific Team.

    However, it seems that the other Abreview ab68538 does not have the “Rating” information and the system recognizes it as a potential problem. I have resent this to you and ask you please look through all the information and resubmit it.

    Thank you for your co-operation and I look forward to receiving your Abreviews.

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    Answer

    DISCOUNT CODES:

    ab19980 - 100%ABR-E76ZR
    ab20556 - 100%ABR-E76ZL
    ab68538 - 100%ABR-E7657




    Expiration date: 12/11/2012

    I am very pleased to hear you would like to accept our offer and testab19980, ab20556 and ab68538in IHC-P.

    This code will give you: 1 per discount code free PRIMARY ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Answer

    Thank you for your response and your interest.

    It is very unusual to offer a discount code for more than 1 product particularly when the application is not one of the standard immunoassays (Western blot, ELISA, Flow cytometry, Immunohistochemistry etc).

    The technique you wish to use/test our products is not the classical IHC-P (formalin-fixed paraffin embedded immunohistochemistry) procedure. At this stage it is not know how frequently other customers will use these products in such an assay and how easy to modify the technique.

    Could you please confirm how the tissue sections will be processed and fixed and whether you have any publication on this specific technique? We need to consider carefully the applicability of this assay.

    I look forward to hearing from you soon.

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    Answer

    I now have more details on experiments done on the antibody ab19980. This antibody has been tested by us only against the immunogen species E. faecium. To the best of my knowledge, I know that there have been experiments done externally that were able to detect E. faecalis, however I do not have access to the assay details. With thanks,

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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