Enterokinase Activity Assay Kit (Fluorometric) (ab170964)

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Overview

  • Product name

    Enterokinase Activity Assay Kit (Fluorometric)
    See all Enterokinase kits

  • Detection method

    Fluorescent

  • Sample type

    Purified protein

  • Assay type

    Enzyme activity (quantitative)

  • Sensitivity

    >= 1 mU/well

  • Species reactivity

    Reacts with: Other species, Mammals

  • Product overview

    Abcam's Enterokinase Activity Assay Kit (Fluorometric) utilizes a peptide substrate containing the Enterokinase recognition sequence along with a fluorescent label ‘AFC’. Enterokinase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enterokinase activity as low as 1 mU.

  • Notes

    Enterokinase (Enteropeptidase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enterokinase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    AFC Standard Yellow 1 x 100µl
    Enteropeptidase Assay Buffer WM 1 x 20ml
    Enteropeptidase Substrate Red 1 x 0.2ml
    Human Enteropeptidase Green 1 x 17µl

  • Research areas

  • Function

    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

  • Tissue specificity

    Intestinal brush border.

  • Involvement in disease

    Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 2 LDL-receptor class A domains.
    Contains 1 MAM domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
    Contains 1 SRCR domain.

  • Post-translational
    modifications

    The chains are derived from a single precursor that is cleaved by a trypsin-like protease.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • Enterokinase
    • Enteropeptidase catalytic light chain
    • ENTK
    • ENTK_HUMAN
    • PRSS7
    • Serine protease 7
    • TMPRSS15
    • Transmembrane protease serine 15
    see all

Images

  • AFC Standard Curve
    AFC Standard Curve
  • Measurement of Enterokinase Levels
    Measurement of Enterokinase Levels

Protocols

References

ab170964 has not yet been referenced specifically in any publications.

Publishing research using ab170964? Please let us know so that we can cite the reference in this datasheet.

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