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    enterokinase-activity-assay-kit-fluorometric-ab170964.pdf

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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Other proteases
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Enterokinase Activity Assay Kit (Fluorometric) (ab170964)

  • Datasheet
  • SDS
  • Protocol Booklet
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AFC Standard Curve
  • Measurement of Enterokinase Levels

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Purified protein
  • Sensitivity: 1 µU

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Overview

  • Product name

    Enterokinase Activity Assay Kit (Fluorometric)
    See all Enterokinase kits
  • Detection method

    Fluorescent
  • Sample type

    Purified protein
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    >= 1 µU
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Abcam's Enterokinase Activity Assay Kit (Fluorometric) utilizes a peptide substrate containing the Enterokinase recognition sequence along with a fluorescent label ‘AFC’. Enterokinase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enterokinase activity as low as 1 mU.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K758 Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit. K758-100 is the same size as the 100 test size of ab170964.

    Enterokinase (Enteropeptidase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enterokinase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    AFC Standard Yellow 1 x 100µl
    Enteropeptidase Assay Buffer WM 1 x 20ml
    Enteropeptidase Substrate Red 1 x 0.2ml
    Human Enteropeptidase Green 1 x 50µl
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
  • Function

    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • Tissue specificity

    Intestinal brush border.
  • Involvement in disease

    Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 2 LDL-receptor class A domains.
    Contains 1 MAM domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
    Contains 1 SRCR domain.
  • Post-translational
    modifications

    The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P98073 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • PRSS 7
    • PRSS7
    • Enterokinase
    • Enterokinase catalytic subunit
    • Enteropeptidase
    • Enteropeptidase catalytic light chain
    • Enteropeptidase non catalytic heavy chain
    • ENTK
    • ENTK_HUMAN
    • PROENTEROKINASE
    • protease, serine, 7 (enterokinase)
    • PRSS7
    • Serine protease 7
    • TMPRSS15
    • Transmembrane protease serine 15
    see all

Images

  • AFC Standard Curve
    AFC Standard Curve
  • Measurement of Enterokinase Levels
    Measurement of Enterokinase Levels

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab170964? Please let us know so that we can cite the reference in this datasheet.

ab170964 has not yet been referenced specifically in any publications.

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