Overview

  • Product name
    Anti-Enterokinase antibody - C-terminal
    See all Enterokinase primary antibodies
  • Description
    Rabbit polyclonal to Enterokinase - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Enterokinase aa 963-995 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P98073

  • Positive control
    • Jurkat cell line lysate; Human stomach tissue; Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 113 kDa.

Target

  • Function
    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • Tissue specificity
    Intestinal brush border.
  • Involvement in disease
    Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 2 LDL-receptor class A domains.
    Contains 1 MAM domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
    Contains 1 SRCR domain.
  • Post-translational
    modifications
    The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • PRSS 7 antibody
    • PRSS7 antibody
    • Enterokinase antibody
    • Enterokinase catalytic subunit antibody
    • Enteropeptidase antibody
    • Enteropeptidase catalytic light chain antibody
    • Enteropeptidase non catalytic heavy chain antibody
    • ENTK antibody
    • ENTK_HUMAN antibody
    • PROENTEROKINASE antibody
    • protease, serine, 7 (enterokinase) antibody
    • PRSS7 antibody
    • Serine protease 7 antibody
    • TMPRSS15 antibody
    • Transmembrane protease serine 15 antibody
    see all

Images

  • Anti-Enterokinase antibody - C-terminal (ab170601) at 1/100 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size: 113 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human stomach tissue labeling Enterokinase with ab170601 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometric analysis of Jurkat cells labeling Enterokinase with ab170601 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab170601 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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