Overview

  • Product name

    Enterokinase cleavage kit
  • Product overview

    Abcam’s Enterokinase cleavage kit (ab207001) efficiently remove tags from recombinant fusion proteins containing accessible enteropeptidase-specific recognition sequence.


    Enteropeptidase (enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine (K) residue. The high specific activity of enteropeptidase is also used to cleave native and fusion proteins containing this recognition motif, and is often used to remove the tag from a recombinant protein. Abcam’s Enterokinase cleavage kit contains a highly active light chain fragment of human enteropeptidase. Our pure enzyme is an excellent tool to obtain a wild type protein sequence from a fusion protein that contains the enteropeptidase recognition sequence. This kit is sufficient for cleaving at least 5 mg of target protein. The residual enteropeptidase left in the reaction mix will not interfere with most experiments that the target protein will be used in. Following cleavage of the target protein, the enteropeptidase may be removed using nickel or cobalt beads if required.

  • Tested applications

    Suitable for: Purificationmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Cleavage Control Protein (hPRL-Trx) (50 μg) 1 vial
    Enteropeptidase Cleavage Buffer 1 x 20ml
    Human Enteropeptidase (100 U) 1 x 34µl

Applications

Our Abpromise guarantee covers the use of ab207001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.

Images

  • SDS-PAGE analysis of the cleavage of hPRL-Trx using different amounts of enteropeptidase. hPRL-Trx (50 µg) was digested into its individual protein fragments hPRL (25.3 kDa) and Trx (17 kDa) using different amounts (0.01-1 U) of enteropeptidase at room temperature for 20 hours.

  • SDS-PAGE analysis of the cleavage of hPRL-Trx by enteropeptidase over time: Analysis of hPRL-Trx digestion at different time points at room temperature using 1 U of enteropeptidase.

Protocols

References

ab207001 has not yet been referenced specifically in any publications.

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