Enterokinase Inhibitor Assay Kit (Fluorometric) (ab170965)
- Datasheet
- References
- Protocols
Overview
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Product name
Enterokinase Inhibitor Assay Kit (Fluorometric)
See all Enterokinase kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Abcam's Enterokinase Inhibitor Screening Kit (Fluorometric) utilizes a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. In the presence of potent Enteropeptidase inhibitor, the hydrolyzation of the substrate will be impeded. The kit provides a rapid, simple and reliable test for screening potential inhibitors of Enteropeptidase.
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Notes
Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Enteropeptidase Assay Buffer WM 1 x 20ml Enteropeptidase Substrate Red 1 x 0.2ml Enteropeptidase-specific Inhibitor [Aprotinin] Blue 1 x 0.1ml Human Enteropeptidase Green 1 x 0.17ml -
Research areas
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Function
Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. -
Tissue specificity
Intestinal brush border. -
Involvement in disease
Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 2 LDL-receptor class A domains.
Contains 1 MAM domain.
Contains 1 peptidase S1 domain.
Contains 1 SEA domain.
Contains 1 SRCR domain. -
Post-translational
modificationsThe chains are derived from a single precursor that is cleaved by a trypsin-like protease. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Enterokinase
- Enteropeptidase catalytic light chain
- ENTK
see all
Images
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Inhibition of EnteropeptidaseInhibition of Enteropeptidase activity by Enteropeptidase-specific Inhibitor, Aprotinin. Assay was performed following kit protocol.
Protocols
References
ab170965 has not yet been referenced specifically in any publications.
