Enterokinase Inhibitor Screening Kit (Fluorometric) (ab170965)

Overview

  • Product name
    Enterokinase Inhibitor Screening Kit (Fluorometric)
    See all Enterokinase kits
  • Sample type
    Inhibitor compounds
  • Assay type
    Enzyme activity
  • Product overview

    Abcam's Enterokinase Inhibitor Screening Kit (Fluorometric) utilizes a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. In the presence of potent Enteropeptidase inhibitor, the hydrolyzation of the substrate will be impeded. The kit provides a rapid, simple and reliable test for screening potential inhibitors of Enteropeptidase.

  • Notes

    Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Enteropeptidase Assay Buffer WM 1 x 20ml
    Enteropeptidase Substrate Red 1 x 0.2ml
    Enteropeptidase-specific Inhibitor [Aprotinin] Blue 1 x 0.1ml
    Human Enteropeptidase Green 1 x 0.17ml
  • Research areas
  • Function
    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • Tissue specificity
    Intestinal brush border.
  • Involvement in disease
    Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 2 LDL-receptor class A domains.
    Contains 1 MAM domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
    Contains 1 SRCR domain.
  • Post-translational
    modifications
    The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • Enterokinase
    • Enteropeptidase catalytic light chain
    • ENTK
    • ENTK_HUMAN
    • PRSS7
    • Serine protease 7
    • TMPRSS15
    • Transmembrane protease serine 15
    see all

Images

  • Inhibition of Enteropeptidase activity by Enteropeptidase-specific Inhibitor, Aprotinin. Assay was performed following kit protocol.

Protocols

References

ab170965 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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