Anti-Enterovirus 71 antibody [10F0] (ab36367)

Thank you for your inquiry.

This antibody is suppliedas ascites and not purified. Ascites contains other proteins in addition tothe specific antibody. Since the antibody concentration is usually measured by measuring protein content it is not possible to supply your customer with the antibody concentration.

We generally expect 5 to 10mg/ml of specific antibody in ascites.

I hope this information is helpful for your customer. Please feel free to contact me again with any further questions.

Thank you very much for your inquiry. I can confirm that the strain BrCr of Enterovirus 71 has been used as an immunogen. I hope this information is useful. Please do not hesitate to contact us should you have any additional question. I wish you all the best.    

Thank you very much for your inquiry. Indeed, this antibody is an ascite and the concentration has not been determined. The concentration is indeed difficult to determine, as an ascite is not a purified antibody and contains other proteins. the concentrations of an antibody in an ascite normally vary from 1 to 10mg/ml. In order to set up the ELISA, I do however not think that the concentration is necessary. Indeed, the antibodies should be used at saturating conditions to obtain reproducible results. This means, that when setting up the ELISA with different concentration of this antibody, a antibody dilution condition should be used where the values are well in the plateau of the curve (meaning well saturating the measure). In order to be able to create a standard curve and quantify therefore the protein of interest, a sample with the known concentration of the protein of interest (in this case Enterovirus 71 proteins) should be used. This can be either the recombinant proteins or a sample with know concentration. In the standard curve, the antibodies should be used in saturating conditions again and the only variable should be the concentration of the protein of interest. I hope this information was helpful. Please let me know if the customer would like me to explain this in more details or if he has any questions or concerns.

DISCOUNT CODE: XXXXXX Expiration date: 22-01-2012 I am very pleased to hear you would like to accept our offer and test ab36367 in Indirect ELISA. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for iELISAand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you very much for your interest in ab36367. To our knowledge, ab36367 has not been tested in Indirect ELISA. Therefore, I can offer a discount off a future purchase if you buy ab36367 now, test it in iELISAand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab36367 in iELISA. I will then send a discount code. This code must be issued before purchasing ab36367 so please wait for my reply before ordering. 2. Purchase ab36367 either by phone, fax, or online (www.abcam.com). 3. Test it in iELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab36367 turns out to be unsuitable for iELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you very much for your inquiry and your patience while we have been inquiring. I have received the information from the laboratory that according to the originators, the clone completely neutralized the ability of the BrCr strain to infect cells (the originators indicate that they used RD and THP-1 cells) at a 1/14 dilution. I hope this information is helpful. Please do not hesitate to contact us again should you need some further information.

Please find below the protocol to use ab36367: The fixation the we would recommend for the use of this product in IF is cold acetone for 10 minutes. The cells will not require further fixation and the recommended dilution is between 1/500 and 1/2000. To dilute the antibody use a proteinaceous buffer pH 7.5 to pH 8.0, eg PBS with 1% BSA.