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  1. Link

    enterovirus-71-antibody-10f0-ab36367.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus Other viruses
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Anti-Enterovirus 71 antibody [10F0] (ab36367)

  • Datasheet
  • SDS
Submit a review Q&A (7)References (15)

Product price, shipping and contact information

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Shipping and order information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Mouse monoclonal [10F0] to Enterovirus 71
  • Suitable for: ICC/IF, Neutralising
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Enterovirus 71 antibody [10F0]
    See all Enterovirus 71 primary antibodies
  • Description

    Mouse monoclonal [10F0] to Enterovirus 71
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Neutralisingmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue, cells or virus corresponding to Enterovirus 71. Native Enterovirus 71

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituent: Ascites
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    10F0
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • Other viruses

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500 - 1/2000.
Fix with cold acetone for 10 minutes. Dilute antibody in PBS (pH 7.5 to pH 8.0) with 1% BSA.
Neutralising
1/14.
Notes
ICC/IF
1/500 - 1/2000.
Fix with cold acetone for 10 minutes. Dilute antibody in PBS (pH 7.5 to pH 8.0) with 1% BSA.
Neutralising
1/14.

Target

  • Relevance

    Enterovirus 71 was first isolated in 1969. Since then it has been associated with sporadic cases of outbreaks in various parts of the world, including the U.S., Brazil, Europe, Austrailia, Malaysia, and Taiwan. Entervirus 71 infection may present itself much like other enterovirus infections, with symptoms ranging from asymptomatic to the presentation of rashes, diarrhea, colds, meningitis, encephalitis, hand to foot to mouth disease, myocarditis, or some combination of these syndromes. On the other hand, Enterovirus 71 may cause many unusal syndromes such as acute flaccid paralysis (similar to paralytic poliomyelitis), bulbar and brain encephalitis, Guillain Barre syndrome, and rapidly fatal pulmonary edema and hemmorrhage.
  • Alternative names

    • EV71 antibody

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (15)

Publishing research using ab36367? Please let us know so that we can cite the reference in this datasheet.

ab36367 has been referenced in 15 publications.

  • Zhang R  et al. DEAD-Box Helicase DDX6 Facilitated RIG-I-Mediated Type-I Interferon Response to EV71 Infection. Front Cell Infect Microbiol 11:725392 (2021). PubMed: 34485180
  • Cosset É  et al. Modeling Poliovirus Infection Using Human Engineered Neural Tissue Enriched With Motor Neuron Derived From Embryonic Stem Cells. Front Cell Dev Biol 8:593106 (2020). PubMed: 33490061
  • Sun L  et al. Intra-host emergence of an enterovirus A71 variant with enhanced PSGL1 usage and neurovirulence. Emerg Microbes Infect 8:1076-1085 (2019). PubMed: 31339457
  • Chen SL  et al. Endophilin-A2-mediated endocytic pathway is critical for enterovirus 71 entry into caco-2 cells. Emerg Microbes Infect 8:773-786 (2019). PubMed: 31132962
  • Wang H  et al. STAT3 Regulates the Type I IFN-Mediated Antiviral Response by Interfering with the Nuclear Entry of STAT1. Int J Mol Sci 20:N/A (2019). PubMed: 31575039
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Question

Dear technical support team: Sorry to disturb you. Our customer raised an enquire about ab36367 (Anti-Enterovirus 71 antibody [10F0]). She would like to know the currently in stock lot number and its corresponding quantity. Would you please help this customer to solve the problem? Thanks for your kindly help Best regards

Read More

Abcam community

Verified customer

Asked on Feb 22 2012

Answer

Thank you for your inquiry.

This antibody is suppliedas ascites and not purified. Ascites contains other proteins in addition tothe specific antibody. Since the antibody concentration is usually measured by measuring protein content it is not possible to supply your customer with the antibody concentration.

We generally expect 5 to 10mg/ml of specific antibody in ascites.

I hope this information is helpful for your customer. Please feel free to contact me again with any further questions.

Read More

Abcam Scientific Support

Answered on Feb 22 2012

Question

    Dear Technical Team,   Hope you are doing fine!   Kindly let us know which strain of Enterovirus 71 was used as an immunogen to develop Anti-Enterovirus 71 antibody [10F0] (ab36367) since on its datasheet it is mentioned as Native Enterovirus 71.   Looking forward towards your valued response on the above.   With Best Regards,    

Read More

Abcam community

Verified customer

Asked on Nov 03 2011

Answer

Thank you very much for your inquiry. I can confirm that the strain BrCr of Enterovirus 71 has been used as an immunogen. I hope this information is useful. Please do not hesitate to contact us should you have any additional question. I wish you all the best.    

Read More

Abcam Scientific Support

Answered on Nov 03 2011

Question

 Dear technical support team: This customer has purchased ab36367 (Anti-Enterovirus 71 antibody [10F0]), and she wants to use this antibody to conduct sELISA and also has a discount code. Therefore, she wants to know the concentration of this antibody, moreover, is there any suggestion can help this customer to create her standard curve if this antibody’s concentration can’t be determined ,could you please help this customer to solve the problem? Thanks for your kindly help. Best regards

Read More

Abcam community

Verified customer

Asked on Oct 18 2011

Answer

Thank you very much for your inquiry. Indeed, this antibody is an ascite and the concentration has not been determined. The concentration is indeed difficult to determine, as an ascite is not a purified antibody and contains other proteins. the concentrations of an antibody in an ascite normally vary from 1 to 10mg/ml. In order to set up the ELISA, I do however not think that the concentration is necessary. Indeed, the antibodies should be used at saturating conditions to obtain reproducible results. This means, that when setting up the ELISA with different concentration of this antibody, a antibody dilution condition should be used where the values are well in the plateau of the curve (meaning well saturating the measure). In order to be able to create a standard curve and quantify therefore the protein of interest, a sample with the known concentration of the protein of interest (in this case Enterovirus 71 proteins) should be used. This can be either the recombinant proteins or a sample with know concentration. In the standard curve, the antibodies should be used in saturating conditions again and the only variable should be the concentration of the protein of interest. I hope this information was helpful. Please let me know if the customer would like me to explain this in more details or if he has any questions or concerns.

Read More

Abcam Scientific Support

Answered on Oct 18 2011

Question

Thanks for your kindly reply, after I contacted with this customer she would like to order ab36367 (Anti-Enterovirus 71 antibody [10F0]) and test in iELISA, would you please help this customer to create a discount code? Thanks for your kindly assistance.  Best regards

Read More

Abcam community

Verified customer

Asked on Sep 23 2011

Answer

DISCOUNT CODE: XXXXXX Expiration date: 22-01-2012 I am very pleased to hear you would like to accept our offer and test ab36367 in Indirect ELISA. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for iELISAand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Sep 23 2011

Question

Dear abcam technical support team: This customer would like to order ab36367 (Anti-Enterovirus 71 antibody [10F0]), this antibody’s datasheets suggest these antibodies only can work in ICC/IF and Neut, but the customer wants to use this antibody conducted the ELISA assay, would you please give this customer some information or suggestion? Thanks for your kindly assistance. Best regards

Read More

Abcam community

Verified customer

Asked on Sep 22 2011

Answer

Thank you very much for your interest in ab36367. To our knowledge, ab36367 has not been tested in Indirect ELISA. Therefore, I can offer a discount off a future purchase if you buy ab36367 now, test it in iELISAand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab36367 in iELISA. I will then send a discount code. This code must be issued before purchasing ab36367 so please wait for my reply before ordering. 2. Purchase ab36367 either by phone, fax, or online (www.abcam.com). 3. Test it in iELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab36367 turns out to be unsuitable for iELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Sep 22 2011

Question

Dear Sir or Madam, on what cells and with which Enterovirus 71 strain was the neutralizing titer of 1/14 given in the data sheet determined? Best regards

Read More

Abcam community

Verified customer

Asked on Sep 01 2011

Answer

Thank you very much for your inquiry and your patience while we have been inquiring. I have received the information from the laboratory that according to the originators, the clone completely neutralized the ability of the BrCr strain to infect cells (the originators indicate that they used RD and THP-1 cells) at a 1/14 dilution. I hope this information is helpful. Please do not hesitate to contact us again should you need some further information.

Read More

Abcam Scientific Support

Answered on Sep 01 2011

Question

Please provide the full ICC protocol for ab36367 and its datasheet.

Read More

Abcam community

Verified customer

Asked on Aug 01 2007

Answer

Please find below the protocol to use ab36367: The fixation the we would recommend for the use of this product in IF is cold acetone for 10 minutes. The cells will not require further fixation and the recommended dilution is between 1/500 and 1/2000. To dilute the antibody use a proteinaceous buffer pH 7.5 to pH 8.0, eg PBS with 1% BSA.

Read More

Abcam Scientific Support

Answered on Aug 01 2007

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