Recombinant Anti-ENTPD5 antibody [EPR3783] (ab92542)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3783] to ENTPD5
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ENTPD5 antibody [EPR3783]
See all ENTPD5 primary antibodies -
Description
Rabbit monoclonal [EPR3783] to ENTPD5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ENTPD5 aa 100-200. The exact sequence is proprietary.
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Positive control
- Human fetal liver, Hela and LNCaP lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3783 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 47 kDa. |
Target
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Function
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. -
Tissue specificity
Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the GDA1/CD39 NTPase family. -
Post-translational
modificationsN-glycosylated; high-mannose type. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 957 Human
- Entrez Gene: 12499 Mouse
- Entrez Gene: 314312 Rat
- Omim: 603162 Human
- SwissProt: O75356 Human
- SwissProt: Q9WUZ9 Mouse
- SwissProt: Q6P6S9 Rat
- Unigene: 720540 Human
see all -
Alternative names
- CD39 like 4 antibody
- AI196558 antibody
- AI987697 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92542 has been referenced in 1 publication.
- Shelton LM et al. Integrated transcriptomic and proteomic analyses uncover regulatory roles of Nrf2 in the kidney. Kidney Int 88:1261-1273 (2015). WB ; Mouse . PubMed: 26422507