Recombinant Anti-ENTPD5 antibody [EPR3783] (ab92542)
![Western blot - Anti-ENTPD5 antibody [EPR3783] (ab92542)](https://www.abcam.com/ps/products/92/ab92542/Images/ENTPD5-Primary-antibodies-ab92542-1.jpg "Western blot - Anti-ENTPD5 antibody [EPR3783] (ab92542)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3783] to ENTPD5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ENTPD5 antibody [EPR3783]
See all ENTPD5 primary antibodies -
Description
Rabbit monoclonal [EPR3783] to ENTPD5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ENTPD5 aa 100-200. The exact sequence is proprietary.
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Positive control
- Human fetal liver, Hela and LNCaP lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3783 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 47 kDa. |
Target
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Function
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. -
Tissue specificity
Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the GDA1/CD39 NTPase family. -
Post-translational
modificationsN-glycosylated; high-mannose type. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 957 Human
- Entrez Gene: 12499 Mouse
- Entrez Gene: 314312 Rat
- Omim: 603162 Human
- SwissProt: O75356 Human
- SwissProt: Q9WUZ9 Mouse
- SwissProt: Q6P6S9 Rat
- Unigene: 720540 Human
see all -
Alternative names
- CD39 like 4 antibody
- AI196558 antibody
- AI987697 antibody
see all
Images
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Western blot - Anti-ENTPD5 antibody [EPR3783] (ab92542)All lanes : Anti-ENTPD5 antibody [EPR3783] (ab92542) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Hela cell lysate
Lane 3 : LNCaP cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 47 kDa -
![Anti-ENTPD5 antibody [EPR3783] (ab92542)](https://www.abcam.com/ps/products/92/ab92542/Images/ab92542-2-benefits-of-recombinant-antibodies.png)
Anti-ENTPD5 antibody [EPR3783] (ab92542)
Datasheets and documents
References (1)
ab92542 has been referenced in 1 publication.
- Shelton LM et al. Integrated transcriptomic and proteomic analyses uncover regulatory roles of Nrf2 in the kidney. Kidney Int 88:1261-1273 (2015). WB ; Mouse . PubMed: 26422507
