Recombinant
Recombinant Anti-ENTPD5 antibody [EPR3783] (ab92542)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ENTPD5 antibody [EPR3783]
See all ENTPD5 primary antibodies -
Description
Rabbit monoclonal [EPR3783] to ENTPD5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ENTPD5 aa 100-200. The exact sequence is proprietary.
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Positive control
- Human fetal liver, Hela and LNCaP lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3783 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 47 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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Function
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. -
Tissue specificity
Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the GDA1/CD39 NTPase family. -
Post-translational
modificationsN-glycosylated; high-mannose type. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 957 Human
- Entrez Gene: 12499 Mouse
- Entrez Gene: 314312 Rat
- Omim: 603162 Human
- SwissProt: O75356 Human
- SwissProt: Q9WUZ9 Mouse
- SwissProt: Q6P6S9 Rat
- Unigene: 720540 Human
see all -
Alternative names
- CD39 like 4 antibody
- AI196558 antibody
- AI987697 antibody
see all
Images
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![Western blot - Anti-ENTPD5 antibody [EPR3783] (ab92542)](https://www.abcam.com/ps/products/92/ab92542/Images/ENTPD5-Primary-antibodies-ab92542-1.jpg)
Western blot - Anti-ENTPD5 antibody [EPR3783] (ab92542)All lanes : Anti-ENTPD5 antibody [EPR3783] (ab92542) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Hela cell lysate
Lane 3 : LNCaP cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 47 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Shelton LM et al. Integrated transcriptomic and proteomic analyses uncover regulatory roles of Nrf2 in the kidney. Kidney Int 88:1261-1273 (2015). WB ; Mouse . Read more (PubMed: 26422507) »
