Overview

  • Product name

    Anti-ENTPD5 antibody [EPR3784]
    See all ENTPD5 primary antibodies
  • Description

    Rabbit monoclonal [EPR3784] to ENTPD5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENTPD5 aa 300-400 (internal sequence). The exact sequence is proprietary.
    (Peptide available as ab190069)

  • Positive control

    • Fetal liver cell lysate; Human kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).Can be blocked with ENTPD5 peptide (ab190069).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
    • Tissue specificity

      Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.
    • Pathway

      Protein modification; protein glycosylation.
    • Sequence similarities

      Belongs to the GDA1/CD39 NTPase family.
    • Post-translational
      modifications

      N-glycosylated; high-mannose type.
    • Cellular localization

      Endoplasmic reticulum lumen.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD39 like 4 antibody
      • AI196558 antibody
      • AI987697 antibody
      • CD39 antigen like 4 antibody
      • CD39 antigen-like 4 antibody
      • CD39 like 4 antibody
      • CD39L4 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 5 antibody
      • ENTP5_HUMAN antibody
      • Entpd5 antibody
      • ER UDPase antibody
      • ER-UDPase antibody
      • GDPase ENTPD5 antibody
      • Guanosine-diphosphatase ENTPD5 antibody
      • MGC163357 antibody
      • MGC163359 antibody
      • mNTPase antibody
      • NTPDase 5 antibody
      • Nucleoside diphosphatase antibody
      • PCPH antibody
      • Pcph proto oncogene protein antibody
      • Proto oncogene CPH antibody
      • UDPase ENTPD5 antibody
      • Uridine-diphosphatase ENTPD5 antibody
      see all

    Images

    • Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution + fetal liver cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 47 kDa
      why is the actual band size different from the predicted?

    • ab108603, at 1/100 dilution, staining ENTPD5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Wei J  et al. ER-associated ubiquitin ligase HRD1 programs liver metabolism by targeting multiple metabolic enzymes. Nat Commun 9:3659 (2018). Read more (PubMed: 30201971) »
    • Walsh J  et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics 108:171-87 (2014). WB ; Mouse . Read more (PubMed: 24859727) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Cell (Spermatozoa)
    Specification
    Spermatozoa
    Permeabilization
    Yes - Triton X 0.25%
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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