Overview

  • Product name
    Eosinophil-Mast Cell Stain Kit (Hematologic Stain)
  • Product overview

    The Combined Eosinophil-Mast Cell (Hematologic Stain) is intended for simultaneous visualization of eosinophils and mast cell.


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Mast Cells    Bright Blue
    Eosinophils      Bright Red
    Nuclei Blue

    Control Tissue: Any well-fixed section (3-5 microns) or any air dried smear on a clean slide.

Properties

Images

  • ab150665 Eosinophil-Mast Cell Stain Kit (Hematologic Stain) staining mast cells and eosinophils in formalin-fixed-paraffin embedded dog mast cell tumor.

  • Combined staining of Eosinophil-Mast cells using ab150665.   

Protocols

References

ab150665 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

good kit for an overview

Good Excellent 5/5 (Ease of Use)
Abreviews
The kit is very easy to use. The staining takes about 30 minutes.
Eosinophils and mast cells can be stained well with this kit and you can see them well. But many other cells and structures are also stained. To identify the cells you are looking for, you need to know the histological structures of eosinophils and mast cells.

Pictures:
1) Skin with eosinophils
2) Skin with mast cells and eosinophils
3) cytospin with one postive eosinophil and negative cells
4) cytospin with isolated peripheral eosinophils (red) and a lymphocyte (blue)

The stains were counterstained with hematoxylin.

Abcam user community

Verified customer

Submitted Feb 06 2018

Answer


Based on our current knowledge, the neutrophils would probably stain a light pink. However, the kit should not be considered specific for only those two cell types (mast cell vs. eosinophil) but does stain those two cell types different colors for easier visualization.

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