Overview

  • Product name
  • Description
    Rabbit polyclonal to Eotaxin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-R, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 83-97 of Human Eotaxin.

  • Positive control
    • The recombinant protein can be used as positive control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    DEAE-Chromatography
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-R Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with Eotaxin peptide (ab8127). Monomer and homodimer are detected at approximate 9 and 18 kDa.

Target

  • Function
    In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 11 antibody
    • CCL 11 antibody
    • CCL11 antibody
    • CCL11_HUMAN antibody
    • chemokine (C-C motif) ligand 11 antibody
    • Chemokine C C motif ligand 11 antibody
    • Chemokine CC Motif Ligand 11 antibody
    • Chemokine ligand 11 antibody
    • Eosinophil chemotactic protein antibody
    • Eotaxin antibody
    • eotaxin-1 antibody
    • MGC22554 antibody
    • SCYA 11 antibody
    • SCYA11 antibody
    • Small inducible cytokine A11 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 11 antibody
    • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) antibody
    • Small inducible cytokine subfamily A member 11 antibody
    • Small-inducible cytokine A11 antibody
    see all

Images

  • ab8018 staining Eotaxin in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). The tissue was fixed in acetone and inhibitors and embedded into GMA resin. Samples were incubated with primary antibody (1/250 in Tris buffered saline) for 18 hours at 4°C. A Biotin-conjugated Pig anti-rabbit polyclonal (1/1200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Eotaxin antibody (ab8018) at 1 µg/ml

    Lane 1 : Hela cell lysate containing 10ng full length recombinant eotaxin
    Lane 2 : Hela cell lysate containing 2.5ng full length recombinant eotaxin
    Lane 3 : Hela cell lysate containing 1ng full length recombinant eotaxin
  • ab8018 at 5µg/ml staining Eotaxin in 293 cells by ICC/IF

  • Immunofluorescence of Eotaxin in 293 cells using ab8018 at 10 ug/ml.

References

This product has been referenced in:
  • Mo FM  et al. Interferon {gamma}-Inducible Protein-10 (IP-10) and Eotaxin as Biomarkers in Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci 51:4226-36 (2010). Read more (PubMed: 20220052) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you very much for your interest in our Eotaxin antibodies.

I would recommend using ab133604 in your experiments because it will be covered under our Abpromise guarantee for use in IHC-P on human samples. If you would like to test ab8018 for IHC-P, I can offer a discount off a future purchase if you buy ab8018 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab8018 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab8018 so please wait for my reply before ordering.

2. Purchase ab8018 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab8018 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (Nasal polyp, tonsil, bronchial biopsies)
Specification
Nasal polyp, tonsil, bronchial biopsies

Abcam user community

Verified customer

Submitted Sep 28 2010

Answer

To date, both antibodies have only been tested in Western Blotting

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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