Overview

  • Product name

    Anti-Eotaxin antibody [EPR5825]
    See all Eotaxin primary antibodies
  • Description

    Rabbit monoclonal [EPR5825] to Eotaxin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human Eotaxin

  • Positive control

    • Eotaxin recombinant protein; Human lung tissue
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5825
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa). Note: tested against Eotaxin recombinant protein
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications

      O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • C-C motif chemokine 11 antibody
      • CCL 11 antibody
      • CCL11 antibody
      • CCL11_HUMAN antibody
      • chemokine (C-C motif) ligand 11 antibody
      • Chemokine C C motif ligand 11 antibody
      • Chemokine CC Motif Ligand 11 antibody
      • Chemokine ligand 11 antibody
      • Eosinophil chemotactic protein antibody
      • Eotaxin antibody
      • eotaxin-1 antibody
      • MGC22554 antibody
      • SCYA 11 antibody
      • SCYA11 antibody
      • Small inducible cytokine A11 antibody
      • Small inducible cytokine subfamily A (Cys Cys) member 11 antibody
      • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) antibody
      • Small inducible cytokine subfamily A member 11 antibody
      • Small-inducible cytokine A11 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Eotaxin with Purified ab133604 at 1:100 dilution (1.21 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/10000 dilution (purified) + Human Eotaxin recombinant protein lysates at 0.015 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 11 kDa
      Observed band size: 8 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST

      Recombinant protein source: Peprotech Inc.
      The region it covers: aa24-aa97(8.3kDa)
      Tag info: N/A

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Eotaxin with unpurified ab133604 at 1/1000.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/1000 dilution (unpurified) + Eotaxin recombinant protein at 0.01 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 11 kDa
      Observed band size: 8 kDa why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Sugimoto M & Kondo M Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress. J Ophthalmol 2016:9292346 (2016). Read more (PubMed: 27340563) »
    • Arnason T  et al. Collagenous gastritis: a morphologic and immunohistochemical study of 40 patients. Mod Pathol N/A:N/A (2014). Read more (PubMed: 25234289) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you very much for your interest in our Eotaxin antibodies.

    I would recommend using ab133604 in your experiments because it will be covered under our Abpromise guarantee for use in IHC-P on human samples. If you would like to test ab8018 for IHC-P, I can offer a discount off a future purchase if you buy ab8018 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab8018 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab8018 so please wait for my reply before ordering.

    2. Purchase ab8018 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-P.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab8018 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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