RecombinantRabMAb

Recombinant Anti-Eotaxin antibody [EPR5825] (ab133604)

Submit a review Q&A (1)References (3)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5825] to Eotaxin
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Eotaxin antibody [EPR5825]
    See all Eotaxin primary antibodies

  • Description

    Rabbit monoclonal [EPR5825] to Eotaxin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to a region within Human Eotaxin

  • Positive control

    • Eotaxin recombinant protein; Human lung tissue

  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5825

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa). Note: tested against Eotaxin recombinant protein
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.

    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.

    • Post-translational
      modifications

      O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • C-C motif chemokine 11 antibody
        • CCL 11 antibody
        • CCL11 antibody
        • CCL11_HUMAN antibody
        • chemokine (C-C motif) ligand 11 antibody
        • Chemokine C C motif ligand 11 antibody
        • Chemokine CC Motif Ligand 11 antibody
        • Chemokine ligand 11 antibody
        • Eosinophil chemotactic protein antibody
        • Eotaxin antibody
        • eotaxin-1 antibody
        • MGC22554 antibody
        • SCYA 11 antibody
        • SCYA11 antibody
        • Small inducible cytokine A11 antibody
        • Small inducible cytokine subfamily A (Cys Cys) member 11 antibody
        • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) antibody
        • Small inducible cytokine subfamily A member 11 antibody
        • Small-inducible cytokine A11 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Eotaxin with Purified ab133604 at 1:100 dilution (1.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      • Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/10000 dilution (purified) + Human Eotaxin recombinant protein lysates at 0.015 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 11 kDa
        Observed band size: 8 kDa
        why is the actual band size different from the predicted?



        Blocking and diluting buffer: 5% NFDM/TBST

        Recombinant protein source: Peprotech Inc.
        The region it covers: aa24-aa97(8.3kDa)
        Tag info: N/A

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)

        Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Eotaxin with unpurified ab133604 at 1/1000.

        Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/1000 dilution (unpurified) + Eotaxin recombinant protein at 0.01 µg

        Secondary
        Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 11 kDa
        Observed band size: 8 kDa why is the actual band size different from the predicted?

      • Other - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Other - Anti-Eotaxin antibody [EPR5825] (ab133604)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References (3)

      Publishing research using ab133604? Please let us know so that we can cite the reference in this datasheet.

      ab133604 has been referenced in 3 publications.

      • Wang R & Huang K CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL-2 and TGF-ß by CD4+ T cells via the STAT5 signaling pathway. Mol Med Rep 21:2522-2532 (2020). PubMed: 32323817
      • Sugimoto M & Kondo M Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress. J Ophthalmol 2016:9292346 (2016). PubMed: 27340563
      • Arnason T  et al. Collagenous gastritis: a morphologic and immunohistochemical study of 40 patients. Mod Pathol N/A:N/A (2014). PubMed: 25234289

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I am interested in studying eotaxin expression in formalin fixed, paraffin embedded human tissue by immunohistochemistry. For this application, one publication is cited for the antibody ab8018 using paraffin embedded tissue. However, your spec sheet suggest that antibody is for resin embedded tissue. The antibody which you market for paraffin embedded tissue is ab133604. For my purpose - IHC in paraffin, would you recommend the ab133604, even though this prior study cited may have used the ab8018 which is opitimized for resin embedded tissue?

      Read More
      Answer

      Thank you very much for your interest in our Eotaxin antibodies.

      I would recommend using ab133604 in your experiments because it will be covered under our Abpromise guarantee for use in IHC-P on human samples. If you would like to test ab8018 for IHC-P, I can offer a discount off a future purchase if you buy ab8018 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab8018 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab8018 so please wait for my reply before ordering.

      2. Purchase ab8018 either by phone, fax, or online (www.abcam.com).

      3. Test it in IHC-P.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab8018 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com