• Product name
  • Description
    Rabbit polyclonal to Epac1
  • Host species
  • Specificity
    ab21236 does not cross react with Epac2. It has not been tested against endogenous Epac1 but recombinant Epac1.
  • Tested applications
    Suitable for: WB, ELISA, IP, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 526-541 of Epac1.

  • Positive control
    • Epac1 recombinant protein
  • General notes
    Ab21236 is supplied in an antibody stabilisation buffer. IgG concentration 0.78-0.94mg/ml in antibody stabilisation buffer.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab21236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 99 kDa.
ELISA 1/10000.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 18577515
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance
    The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
  • Cellular localization
    Endomembrane system
  • Database links
  • Alternative names
    • bcm910 antibody
    • CAMP GEFI antibody
    • cAMP regulated guanine nucleotide exchange factor I antibody
    • CAMPGEFI antibody
    • CGEF 1 antibody
    • CGEF1 antibody
    • EPA1 antibody
    • Epac 1 antibody
    • EPAC antibody
    • EPAC1 antibody
    • Exchange factor directly activated by cAMP 1 antibody
    • Exchange protein directly activated by cAMP 1 antibody
    • MGC21410 antibody
    • RAP guanine nucleotide exchange factor antibody
    • Rap guanine nucleotide exchange factor (GEF) 3 antibody
    • RAP guanine nucleotide exchange factor 3 antibody
    • Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
    • RAPGEF3 antibody
    see all


  • ab21236 staining Epac1 in mouse pancreatic acini tissue section by Immunohistochemistry (Frozen tissue sections). Tissue underwent fixation in 4% paraformaldehyde in PBS pH 7.4 for 30 minutes, PBS washed, cryoprotected and frozen with isopenatane cooled with liquid nitrogen. The primary antibody was diluted 1/200 and incubated with sample for 2 hours at room temperature. A cy3-conjugated donkey polyclonal to rabbit IgG at 1/200 dilution was used as secondary for 1 hour. Prolong Gold with 4,6-diamino-2-phenylindole was added to mounting medium to counterstain nuclei. 

  • ICC/IF image of ab21236 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21236, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Garcia-Morales V  et al. The microRNA-7-mediated reduction in EPAC-1 contributes to vascular endothelial permeability and eNOS uncoupling in murine experimental retinopathy. Acta Diabetol 54:581-591 (2017). Read more (PubMed: 28353063) »
  • Tobin GA  et al. The Role of eNOS Phosphorylation in Causing Drug-induced Vascular Injury. Toxicol Pathol 42:709-724 (2014). Read more (PubMed: 24705881) »

See all 9 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam earlier today.
The composition of antibody stabilization buffer has the following components: the exact concentrations of these constituents is proprietary in nature and cannot be disclosed.
Tris/Glycine buffer ...

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The rabbit monoclonal will be effective for IF. The application field lists ICC (iimunocytochemistry) which is IF (immunofluorescence) if one is staining cell cultures with a fluorochrome.

I have issued a free of charge replacement with ab1...

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Thank you for your thorough reply. One other question I meant to ask was how much sample you load per lane of the gel. We recommend at least 20ug per lane, especially if no signal is evident.

Assuming you are loading enough sample, I suggest...

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Thank you for bringing this to our attention. I am sorry to read that the antibody is giving you unexpected results in these two applications.

There may be a modification I can suggest to improve your result but it may be the case that, as y...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rabbit Cell lysate - whole cell (pancreatic acini)
Loading amount
20 µg
pancreatic acini
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. maria sabbatini

Verified customer

Submitted Sep 09 2008

I'm very sorry for the delay in replying to you. I have now received feedback from the laboratory that tested the antibody ab21236 and was informed that it was tested against recombinant Epac 1 and gave a very good strong band. However, it had poor aff...

Read More
Western blot
Mouse Cell lysate - whole cell (endothelial cells, and liver)
Loading amount
10 µg
endothelial cells, and liver
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3

Abcam user community

Verified customer

Submitted Feb 02 2007


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