Key features and details
- Rabbit polyclonal to Epac1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Epac1 antibody
See all Epac1 primary antibodies
DescriptionRabbit polyclonal to Epac1
Specificityab21236 does not cross react with Epac2. It has not been tested against endogenous Epac1 but recombinant Epac1.
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
General notesAb21236 is supplied in an antibody stabilisation buffer. IgG concentration 0.78-0.94mg/ml in antibody stabilisation buffer.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 5 µg/ml.
RelevanceThe activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
Cellular localizationEndomembrane system
- bcm910 antibody
- CAMP GEFI antibody
- cAMP regulated guanine nucleotide exchange factor I antibody
ICC/IF image of ab21236 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21236, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab21236 has been referenced in 11 publications.
- Silva I et al. ß3 Adrenoceptor-induced cholinergic inhibition in human and rat urinary bladders involves the exchange protein directly activated by cyclic AMP 1 favoring adenosine release. Br J Pharmacol 177:1589-1608 (2020). PubMed: 31721163
- Sun Q et al. Epac1 is involved in cell cycle progression in lung cancer through PKC and Cx43 regulation. Folia Histochem Cytobiol 56:21-26 (2018). PubMed: 29528086
- Garcia-Morales V et al. The microRNA-7-mediated reduction in EPAC-1 contributes to vascular endothelial permeability and eNOS uncoupling in murine experimental retinopathy. Acta Diabetol 54:581-591 (2017). PubMed: 28353063
- Tobin GA et al. The Role of eNOS Phosphorylation in Causing Drug-induced Vascular Injury. Toxicol Pathol 42:709-724 (2014). PubMed: 24705881
- Guibinga GH et al. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22:4502-15 (2013). PubMed: 23804752