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We use 25ug of protein per lane of the gel.
I'd be interested in trying the rabbit monoclonal. Will I only be able to use this for wesstern blots though and not immunofluorescence? For my studies, westerns would be the preferred method of identifying Epac1, but the original antibody gave me the option of doing immunofluorescence.
Asked on Mar 28 2012
The rabbit monoclonal will be effective for IF. The application field lists ICC (iimunocytochemistry) which is IF (immunofluorescence) if one is staining cell cultures with a fluorochrome.
I have issued a free of charge replacement with ab109415.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
Answered on Mar 28 2012