Recombinant
RabMAb

Recombinant Anti-Epac1 antibody [EPR1672] - BSA and Azide free (ab239977)

Overview

  • Product name

    Anti-Epac1 antibody [EPR1672] - BSA and Azide free
    See all Epac1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1672] to Epac1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Epac1 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab239977 is the carrier-free version of ab109415. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239977 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1672
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab239977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended.

 

IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 104 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance

      The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
    • Cellular localization

      Endomembrane system
    • Database links

    • Alternative names

      • bcm910 antibody
      • CAMP GEFI antibody
      • cAMP regulated guanine nucleotide exchange factor I antibody
      • CAMPGEFI antibody
      • CGEF 1 antibody
      • CGEF1 antibody
      • EPA1 antibody
      • Epac 1 antibody
      • EPAC antibody
      • EPAC1 antibody
      • Exchange factor directly activated by cAMP 1 antibody
      • Exchange protein directly activated by cAMP 1 antibody
      • MGC21410 antibody
      • RAP guanine nucleotide exchange factor antibody
      • Rap guanine nucleotide exchange factor (GEF) 3 antibody
      • RAP guanine nucleotide exchange factor 3 antibody
      • Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
      • RAPGEF3 antibody
      see all

    Images

    • Immunohistochemical analysis of Epac1 in paraffin-embedded Human kidney tissue using ab109415 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109415).

    References

    ab239977 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239977.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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