Key features and details
- Rabbit polyclonal to Epac2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Epac2 antibody
See all Epac2 primary antibodies
DescriptionRabbit polyclonal to Epac2
SpecificityAt least two isoforms of Epac2 are known to exist; this antibody will detect only the larger isoform.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 15 amino acids near the N-terminus of Human Epac2 (NP_008954).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 116 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
FunctionGuanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
Tissue specificityPredominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
Sequence similaritiesContains 2 cyclic nucleotide-binding domains.
Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.
DomainThe N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
The DEP domain is involved in membrane localization independent from regulation by cAMP.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
- CAMP GEFII antibody
- cAMP regulated guanine nucleotide exchange factor II antibody
- cAMP-GEFII antibody
All lanes : Anti-Epac2 antibody (ab124189) at 1 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat liver tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 116 kDa
Immunofluorescence of Epac2 in mouse liver tissue with ab124189 at 20 ug/mL.
Immunohistochemistry of EPAC2 in mouse liver tissue with ab124189 at 2.5 ug/mL.
ab124189 has been referenced in 2 publications.
- Trogden KP et al. Regulation of Glucose-Dependent Golgi-Derived Microtubules by cAMP/EPAC2 Promotes Secretory Vesicle Biogenesis in Pancreatic ß Cells. Curr Biol 29:2339-2350.e5 (2019). PubMed: 31303487
- Wang SY et al. Sex Steroids Influence Brain-Derived Neurotropic Factor Secretion From Human Airway Smooth Muscle Cells. J Cell Physiol 231:1586-92 (2016). PubMed: 26566264