Overview

  • Product name
  • Description
    Rabbit polyclonal to Epac2
  • Host species
    Rabbit
  • Specificity
    This antibody does not cross react with other Epac family proteins.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide:

    CPREQFDSLTPLPEQEGPT

    , corresponding to amino acids 743-762 of Epac2

Properties

Applications

Our Abpromise guarantee covers the use of ab21238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 112 kDa.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration. PubMed: 21799930

Target

  • Function
    Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
  • Tissue specificity
    Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
  • Sequence similarities
    Contains 2 cyclic nucleotide-binding domains.
    Contains 1 DEP domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • Domain
    The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
    The DEP domain is involved in membrane localization independent from regulation by cAMP.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAMP GEFII antibody
    • cAMP regulated guanine nucleotide exchange factor II antibody
    • cAMP-GEFII antibody
    • cAMP-regulated guanine nucleotide exchange factor II antibody
    • CAMPGEFII antibody
    • CGEF 2 antibody
    • CGEF2 antibody
    • Epac 2 antibody
    • EPAC2 antibody
    • Exchange factor directly activated by cAMP 2 antibody
    • Exchange protein directly activated by cAMP 2 antibody
    • Nbla00496 antibody
    • Putative protein product of Nbla00496 antibody
    • Rap guanine nucleotide exchange factor (GEF) 4 antibody
    • Rap guanine nucleotide exchange factor 4 antibody
    • RAPGEF 4 antibody
    • Rapgef4 antibody
    • RPGF4_HUMAN antibody
    see all

Images

  • ab21238 staining Epac2 in the bronchial surface epithelial 16HBE14o- cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were rinsed twice with phosphate-buffered saline (PBS) and then fixed with 4% paraformaldehyde for 30 minutes at 4°C, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes and blocked with 10% fetal calf serum (FCS) in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 10% FCS) overnight at 4°C. An Alex Fluor®568-conjugated Goat anti-rabbit IgG was used as the secondary antibody. The corresponding image taken in brightfield is shown on the right.

  • Anti-Epac2 antibody (ab21238) at 1/1000 dilution + Whole cell lysates prepared from rabbit brain (positive control) and kidney, pancreas and pancretic acini (negative controls) at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG

    Performed under reducing conditions.

    Predicted band size: 112 kDa


    Exposure time: 3 minutes

    See Abreview

References

This product has been referenced in:
  • Mostafavi H  et al. Selective ß2 adrenergic agonist increases Cx43 and miR-451 expression via cAMP-Epac. Mol Med Rep 9:2405-10 (2014). Human . Read more (PubMed: 24714982) »
  • Guibinga GH  et al. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22:4502-15 (2013). Read more (PubMed: 23804752) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for providing that information.

Both the Abreview and the reference that are on the webpage for ab21238 use 5% BSA as a blocking agent and I am wondering if using that rather than 5% milk will help get a signal.

I would advise switching to BSA blocking, use a 1/250 primary antibody concentration and also if possible increase the total amount of protein loaded into the wells. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

Please let me know if there is anything else I can help you with.

Read More

Answer

Thank you for contacting Abcam.

I am sorry that you are having problems with ab21238 in western blot. To be able to help you more with this problem, I was wondering if you could give me a little more information:

1- How much sample are you loading (ug)?

2- What type of blocking agent are you using?

3- Apart from 1/500 what primary antibody concentrations have you tried and how long do you incubate for?

We do take all complaints on products seriously and we do our best to solve these problems. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

I look forward to your reply.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (pancreatic acini)
Loading amount
20 µg
Specification
pancreatic acini
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. maria sabbatini

Verified customer

Submitted Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up