• Product name
  • Description
    Rabbit polyclonal to Epac2
  • Host species
  • Specificity
    This antibody does not cross react with other Epac family proteins.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 743-762 of Epac2



Our Abpromise guarantee covers the use of ab21238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 112 kDa.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration. PubMed: 21799930


  • Function
    Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
  • Tissue specificity
    Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
  • Sequence similarities
    Contains 2 cyclic nucleotide-binding domains.
    Contains 1 DEP domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • Domain
    The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
    The DEP domain is involved in membrane localization independent from regulation by cAMP.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAMP GEFII antibody
    • cAMP regulated guanine nucleotide exchange factor II antibody
    • cAMP-GEFII antibody
    • cAMP-regulated guanine nucleotide exchange factor II antibody
    • CAMPGEFII antibody
    • CGEF 2 antibody
    • CGEF2 antibody
    • Epac 2 antibody
    • EPAC2 antibody
    • Exchange factor directly activated by cAMP 2 antibody
    • Exchange protein directly activated by cAMP 2 antibody
    • Nbla00496 antibody
    • Putative protein product of Nbla00496 antibody
    • Rap guanine nucleotide exchange factor (GEF) 4 antibody
    • Rap guanine nucleotide exchange factor 4 antibody
    • RAPGEF 4 antibody
    • Rapgef4 antibody
    • RPGF4_HUMAN antibody
    see all


  • ab21238 staining Epac2 in the bronchial surface epithelial 16HBE14o- cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were rinsed twice with phosphate-buffered saline (PBS) and then fixed with 4% paraformaldehyde for 30 minutes at 4°C, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes and blocked with 10% fetal calf serum (FCS) in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 10% FCS) overnight at 4°C. An Alex Fluor®568-conjugated Goat anti-rabbit IgG was used as the secondary antibody. The corresponding image taken in brightfield is shown on the right.

  • Anti-Epac2 antibody (ab21238) at 1/1000 dilution + Whole cell lysates prepared from rabbit brain (positive control) and kidney, pancreas and pancretic acini (negative controls) at 20 µg

    HRP-conjugated goat anti-rabbit IgG

    Performed under reducing conditions.

    Predicted band size: 112 kDa

    Exposure time: 3 minutes

    See Abreview


This product has been referenced in:
  • Mostafavi H  et al. Selective ß2 adrenergic agonist increases Cx43 and miR-451 expression via cAMP-Epac. Mol Med Rep 9:2405-10 (2014). Human . Read more (PubMed: 24714982) »
  • Guibinga GH  et al. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22:4502-15 (2013). Read more (PubMed: 23804752) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for providing that information.

Both the Abreview and the reference that are on the webpage for ab21238 use 5% BSA as a blocking agent and I am wondering if using that rather than 5% milk will help get a signal.

I would ad...

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Thank you for contacting Abcam.

I am sorry that you are having problems with ab21238 in western blot. To be able to help you more with this problem, I was wondering if you could give me a little more information:

1- How much sample ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rabbit Cell lysate - whole cell (pancreatic acini)
Loading amount
20 µg
pancreatic acini
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. maria sabbatini

Verified customer

Submitted Sep 09 2008


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