Anti-EPB41L1 antibody (ab244499)
Key features and details
- Rabbit polyclonal to EPB41L1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EPB41L1 antibody
See all EPB41L1 primary antibodies -
Description
Rabbit polyclonal to EPB41L1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EPB41L1 aa 600-750.
Database link: Q9H4G0 -
Positive control
- WB:RT4 cell lysate. IHC-P: Human cerebral cortex, adrenal gland, kidney and colon tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
May function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases. -
Tissue specificity
Highest expression in brain, lower in heart, kidney, pancreas, placenta, lung and skeletal muscle. -
Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 2036 Human
- Omim: 602879 Human
- SwissProt: Q9H4G0 Human
- Unigene: 722186 Human
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Alternative names
- 4.1N antibody
- Band 4.1 like protein 1 antibody
- Band 4.1-like protein 1 antibody
see all
Images
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All lanes : Anti-EPB41L1 antibody (ab244499) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 99 kDa -
Paraffin-embedded human colon tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human adrenal gland tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human skeletal muscle tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis. Low expression, as expected.
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PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for EPB41L1 (green) using ab244499 at 4 μg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244499 has not yet been referenced specifically in any publications.