Key features and details
- Rabbit polyclonal to EPB41L1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EPB41L1 antibody
See all EPB41L1 primary antibodies
DescriptionRabbit polyclonal to EPB41L1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB:RT4 cell lysate. IHC-P: Human cerebral cortex, adrenal gland, kidney and colon tissue. ICC/IF: A549 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionMay function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases.
Tissue specificityHighest expression in brain, lower in heart, kidney, pancreas, placenta, lung and skeletal muscle.
Sequence similaritiesContains 1 FERM domain.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- 4.1N antibody
- Band 4.1 like protein 1 antibody
- Band 4.1-like protein 1 antibody
All lanes : Anti-EPB41L1 antibody (ab244499) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 99 kDa
Paraffin-embedded human colon tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis. Low expression, as expected.
PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for EPB41L1 (green) using ab244499 at 4 μg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244499 has not yet been referenced specifically in any publications.