Recombinant Anti-EPB41L1 antibody [EPR11090] (ab179453)
![Western blot - Anti-EPB41L1 antibody [EPR11090] (ab179453)](https://www.abcam.com/ps/products/179/ab179453/Images/ab179453-207476-ab179453.jpg "Western blot - Anti-EPB41L1 antibody [EPR11090] (ab179453)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11090] to EPB41L1
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-EPB41L1 antibody [EPR11090]
See all EPB41L1 primary antibodies -
Description
Rabbit monoclonal [EPR11090] to EPB41L1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human EPB41L1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H4G0 -
Positive control
- Human fetal brain and Neuro 2a lysates; Neuro 2a cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11090 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab179453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 99 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
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| IP | 1/10 - 1/100. |
Target
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Function
May function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases. -
Tissue specificity
Highest expression in brain, lower in heart, kidney, pancreas, placenta, lung and skeletal muscle. -
Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 2036 Human
- Entrez Gene: 13821 Mouse
- Omim: 602879 Human
- SwissProt: Q9H4G0 Human
- SwissProt: Q9Z2H5 Mouse
- Unigene: 437422 Human
- Unigene: 722186 Human
- Unigene: 20852 Mouse
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Alternative names
- 4.1N antibody
- Band 4.1 like protein 1 antibody
- Band 4.1-like protein 1 antibody
see all
Images
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Western blot - Anti-EPB41L1 antibody [EPR11090] (ab179453)All lanes : Anti-EPB41L1 antibody [EPR11090] (ab179453) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Neuro 2a lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
![Flow Cytometry - Anti-EPB41L1 antibody [EPR11090] (ab179453)](https://www.abcam.com/ps/products/179/ab179453/Images/ab179453-207477-ab179453b.jpg)
Flow Cytometry - Anti-EPB41L1 antibody [EPR11090] (ab179453)Flow cytometric analysis of permeabilized Neuro 2a cells labeling EPB41L1 with ab179453 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
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![Immunoprecipitation - Anti-EPB41L1 antibody [EPR11090] (ab179453)](https://www.abcam.com/ps/products/179/ab179453/Images/ab179453-207478-ab179453c.jpg)
Immunoprecipitation - Anti-EPB41L1 antibody [EPR11090] (ab179453)Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab179453 at 1/10 dilution.
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![Anti-EPB41L1 antibody [EPR11090] (ab179453)](https://www.abcam.com/ps/products/179/ab179453/Images/ab179453-4-benefits-of-recombinant-antibodies.png)
Anti-EPB41L1 antibody [EPR11090] (ab179453)
Protocols
Datasheets and documents
References (0)
ab179453 has not yet been referenced specifically in any publications.
