Recombinant Anti-EPB41L1 antibody [EPR11090] (ab179453)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-EPB41L1 antibody [EPR11090]
See all EPB41L1 primary antibodies -
Description
Rabbit monoclonal [EPR11090] to EPB41L1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human EPB41L1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H4G0 -
Positive control
- Human fetal brain and Neuro 2a lysates; Neuro 2a cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11090 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab179453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 99 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IP | 1/10 - 1/100. |
Target
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Function
May function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases. -
Tissue specificity
Highest expression in brain, lower in heart, kidney, pancreas, placenta, lung and skeletal muscle. -
Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 2036 Human
- Entrez Gene: 13821 Mouse
- Omim: 602879 Human
- SwissProt: Q9H4G0 Human
- SwissProt: Q9Z2H5 Mouse
- Unigene: 437422 Human
- Unigene: 722186 Human
- Unigene: 20852 Mouse
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Alternative names
- 4.1N antibody
- Band 4.1 like protein 1 antibody
- Band 4.1-like protein 1 antibody
see all
Images
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![Western blot - Anti-EPB41L1 antibody [EPR11090] (ab179453)](https://www.abcam.com/ps/products/179/ab179453/Images/ab179453-207476-ab179453.jpg)
Western blot - Anti-EPB41L1 antibody [EPR11090] (ab179453)All lanes : Anti-EPB41L1 antibody [EPR11090] (ab179453) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Neuro 2a lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
![Flow Cytometry - Anti-EPB41L1 antibody [EPR11090] (ab179453)](https://www.abcam.com/ps/products/179/ab179453/Images/ab179453-207477-ab179453b.jpg)
Flow Cytometry - Anti-EPB41L1 antibody [EPR11090] (ab179453)Flow cytometric analysis of permeabilized Neuro 2a cells labeling EPB41L1 with ab179453 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
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![Immunoprecipitation - Anti-EPB41L1 antibody [EPR11090] (ab179453)](https://www.abcam.com/ps/products/179/ab179453/Images/ab179453-207478-ab179453c.jpg)
Immunoprecipitation - Anti-EPB41L1 antibody [EPR11090] (ab179453)Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab179453 at 1/10 dilution.
Datasheets and documents
References
ab179453 has not yet been referenced specifically in any publications.
