Recombinant
RabMAb

Recombinant Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)

Overview

  • Product name

    Anti-EPB41L2 antibody [EPR8873(2)]
    See all EPB41L2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8873(2)] to EPB41L2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human EPB41L2 aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O43491

  • Positive control

    • C6, Hela, SH-SY5Y, HepG2 and fetal brain lysates. Human uterus tissue, HepG2 cells
  • General notes

     

     

     This product was previously labelled as EPB4IL2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8873(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 113 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Contains 1 FERM domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4.1 G antibody
      • 4.1G antibody
      • AW555191 antibody
      • Band 4.1 like protein 2 antibody
      • Band 4.1-like protein 2 antibody
      • D10Ertd398e antibody
      • DKFZp781D1972 antibody
      • DKFZp781H1755 antibody
      • E41L2_HUMAN antibody
      • Epb41l2 antibody
      • Erythrocyte membrane protein band 4.1 like 2 antibody
      • Erythrocyte membrane protein band 4.1 like protein 2 antibody
      • Generally expressed protein 4.1 antibody
      • NBL2 antibody
      • OTTHUMP00000017197 antibody
      • OTTHUMP00000040262 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling EPB41L2 with ab17928 at 1/50 dilution.

    • All lanes : Anti-EPB41L2 antibody [EPR8873(2)] (ab175928) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : SH-SH5Y cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Fetal brain cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 113 kDa

    • Immunofluorescent staining of HepG2 cells using labeling EPB41L2 with ab175928 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab175928 at 1/10 dilution

    References

    ab175928 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab175928.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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