Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8873(2)] to EPB41L2
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-EPB41L2 antibody [EPR8873(2)]
See all EPB41L2 primary antibodies
DescriptionRabbit monoclonal [EPR8873(2)] to EPB41L2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
within Human EPB41L2 aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O43491
- C6, Hela, SH-SY5Y, HepG2 and fetal brain lysates. Human uterus tissue, HepG2 cells
This product was previously labelled as EPB4IL2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 113 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 FERM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- 4.1 G antibody
- 4.1G antibody
- AW555191 antibody
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling EPB41L2 with ab17928 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-EPB41L2 antibody [EPR8873(2)] (ab175928) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : SH-SH5Y cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 113 kDa
Immunofluorescent staining of HepG2 cells using labeling EPB41L2 with ab175928 at 1/100 dilution (green). DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab175928 at 1/10 dilution
ab175928 has not yet been referenced specifically in any publications.